Summary of Study ST004431

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002673. The data can be accessed directly via it's Project DOI: 10.21228/M84P1D This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST004431
Study Title	Lipidomic analysis of human abdominal and gluteal white adipose tissue depots
Study Summary	White adipose tissue samples were obtained from five healthy female donors (mean age ± SD, 36.6 ± 8.6 years; BMI ± SD, 26.3 ± 1.4 kg/m²). Tissue samples were collected from both abdominal and gluteal depots during elective surgical procedures.
Institute	University of Szeged
Last Name	David
First Name	Kovacs
Address	Dóm tér 9, Szeged, Csongrád-Csanád, 6723, Hungary
Email	kovacs.david@med.u-szeged.hu
Phone	+3662342665
Submit Date	2025-12-09
Raw Data Available	Yes
Raw Data File Type(s)	mzML, raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2026-01-02
Release Version	1

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Project:

Project ID:	PR002673
Project DOI:	doi: 10.21228/M84P1D
Project Title:	Chain length-dependent mobilization and oxidation of fatty acids in adipocytes
Project Summary:	Fatty acids (FAs) are essential metabolites in energy homeostasis. Adipocytes store FAs as triacylglycerol (TG) in their lipid droplets and mobilize them upon demand in order to provide energy for peripheral tissues. While the mobilization of long-chain fatty acids (LCFAs) during lipolysis is largely documented, there is little information on the metabolism of short- and medium-chain fatty acids (SMCFA) in adipocytes. We demonstrate that adipocytes store SMCFAs in their lipid droplets and that following lipolysis initiation, TGs containing SMCFAs undergo rapid hydrolysis. We found that this process is facilitated by the preferential accumulation of SMCFA-containing TGs at the surface of lipid droplets, thereby enhancing their accessibility to lipases. Unlike LCFAs, SMCFAs are not released from the adipocytes following lipolysis but undergo oxidation within the cell. Our findings suggest that SMCFAs are preferentially mobilized and oxidized to provide energy for the adipocyte, highlighting a distinct metabolic fate compared to LCFAs.
Institute:	University of Szeged
Department:	Institute of Biochemistry
Last Name:	Kovacs
First Name:	David
Address:	Dóm tér 9, Szeged, Csongrád-Csanád, 6723, Hungary
Email:	kovacs.david@med.u-szeged.hu
Phone:	+3662342665

Subject:

Subject ID:	SU004592
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Female

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id	local_sample_id	Extraction phase	Injection order	Sample source
SA523788	9_T-MM_ACN_Pos1	ACN	1	Abdominal WAT
SA523789	7_T-MM_ACN_Pos1	ACN	1	Abdominal WAT
SA523790	1_T-MM_ACN_Pos1	ACN	1	Abdominal WAT
SA523791	5_T-MM_ACN_Pos1	ACN	1	Abdominal WAT
SA523792	3_T-MM_ACN_Pos1	ACN	1	Abdominal WAT
SA523793	2_T-MM_ACN_Pos1	ACN	1	Gluteal WAT
SA523794	8_T-MM_ACN_Pos1	ACN	1	Gluteal WAT
SA523795	6_T-MM_ACN_Pos1	ACN	1	Gluteal WAT
SA523796	4_T-MM_ACN_Pos1	ACN	1	Gluteal WAT
SA523797	10_T-MM_ACN_Pos1	ACN	1	Gluteal WAT
SA523798	5_T-MM_ACN_Pos2	ACN	2	Abdominal WAT
SA523799	7_T-MM_ACN_Pos2	ACN	2	Abdominal WAT
SA523800	9_T-MM_ACN_Pos2	ACN	2	Abdominal WAT
SA523801	3_T-MM_ACN_Pos2	ACN	2	Abdominal WAT
SA523802	1_T-MM_ACN_Pos2	ACN	2	Abdominal WAT
SA523803	4_T-MM_ACN_Pos2	ACN	2	Gluteal WAT
SA523804	2_T-MM_ACN_Pos2	ACN	2	Gluteal WAT
SA523805	8_T-MM_ACN_Pos2	ACN	2	Gluteal WAT
SA523806	6_T-MM_ACN_Pos2	ACN	2	Gluteal WAT
SA523807	10_T-MM_ACN_Pos2	ACN	2	Gluteal WAT
SA523808	7_T-MM_ACN_Pos3	ACN	3	Abdominal WAT
SA523809	9_T-MM_ACN_Pos3	ACN	3	Abdominal WAT
SA523810	5_T-MM_ACN_Pos3	ACN	3	Abdominal WAT
SA523811	1_T-MM_ACN_Pos3	ACN	3	Abdominal WAT
SA523812	4_T-MM_ACN_Pos3	ACN	3	Gluteal WAT
SA523813	8_T-MM_ACN_Pos3	ACN	3	Gluteal WAT
SA523814	2_T-MM_ACN_Pos3	ACN	3	Gluteal WAT
SA523815	10_T-MM_ACN_Pos3	ACN	3	Gluteal WAT
SA523816	6_T-MM_ACN_Pos3	ACN	3	Gluteal WAT
SA523817	5_T-MM_HEX_Pos1	HEX	1	Abdominal WAT
SA523818	3_T-MM_HEX_Pos1	HEX	1	Abdominal WAT
SA523819	1_T-MM_HEX_Pos1	HEX	1	Abdominal WAT
SA523820	9_T-MM_HEX_Pos1	HEX	1	Abdominal WAT
SA523821	7_T-MM_HEX_Pos1	HEX	1	Abdominal WAT
SA523822	8_T-MM_HEX_Pos1	HEX	1	Gluteal WAT
SA523823	2_T-MM_HEX_Pos1	HEX	1	Gluteal WAT
SA523824	4_T-MM_HEX_Pos1	HEX	1	Gluteal WAT
SA523825	10_T-MM_HEX_Pos1	HEX	1	Gluteal WAT
SA523826	6_T-MM_HEX_Pos1	HEX	1	Gluteal WAT
SA523827	7_T-MM_HEX_Pos2	HEX	2	Abdominal WAT
SA523828	3_T-MM_HEX_Pos2	HEX	2	Abdominal WAT
SA523829	9_T-MM_HEX_Pos2	HEX	2	Abdominal WAT
SA523830	1_T-MM_HEX_Pos2	HEX	2	Abdominal WAT
SA523831	5_T-MM_HEX_Pos2	HEX	2	Abdominal WAT
SA523832	2_T-MM_HEX_Pos2	HEX	2	Gluteal WAT
SA523833	10_T-MM_HEX_Pos2	HEX	2	Gluteal WAT
SA523834	8_T-MM_HEX_Pos2	HEX	2	Gluteal WAT
SA523835	6_T-MM_HEX_Pos2	HEX	2	Gluteal WAT
SA523836	4_T-MM_HEX_Pos2	HEX	2	Gluteal WAT
SA523837	9_T-MM_HEX_Pos3	HEX	3	Abdominal WAT
SA523838	1_T-MM_HEX_Pos3	HEX	3	Abdominal WAT
SA523839	7_T-MM_HEX_Pos3	HEX	3	Abdominal WAT
SA523840	5_T-MM_HEX_Pos3	HEX	3	Abdominal WAT
SA523841	3_T-MM_HEX_Pos3	HEX	3	Abdominal WAT
SA523842	8_T-MM_HEX_Pos3	HEX	3	Gluteal WAT
SA523843	6_T-MM_HEX_Pos3	HEX	3	Gluteal WAT
SA523844	4_T-MM_HEX_Pos3	HEX	3	Gluteal WAT
SA523845	2_T-MM_HEX_Pos3	HEX	3	Gluteal WAT
SA523846	10_T-MM_HEX_Pos3	HEX	3	Gluteal WAT

Showing results 1 to 59 of 59

Showing results 1 to 59 of 59

Collection:

Collection ID:	CO004585
Collection Summary:	WAT samples were obtained from five healthy female donors (mean age ± SD, 36.6 ± 8.6 years; BMI ± SD, 26.3 ± 1.4 kg/m²). Tissue samples were collected from both abdominal and gluteal depots during elective surgical procedures. All participants provided written consent prior to inclusion in the study. Sample collection and use for research were approved by the appropriate institutional ethics committee. Samples were stored at -80°C until lipid extractions.
Sample Type:	Adipose tissue
Storage Conditions:	-80℃

Treatment:

Treatment ID:	TR004601
Treatment Summary:	For lipidomics analysis, paired tissue samples obtained from abdominal and gluteal adipose tissue depots were used.

Sample Preparation:

Sampleprep ID:	SP004598
Sampleprep Summary:	To extract lipids from tissue samples, a 3-phase liquid extraction was performed to separate neutral and polar lipids. First, tissue samples were measured on an analytical scale then transferred into glass tubes, containing already the internal standard mix (Splash) and TG 6:0/6:0/6:0. Then, to reduce sample loss, 0.75 mL of ACN were added to microtubes, vortexed, and transferred into the corresponding glass tubes. The remaining solvents were added to each sample: 0.75 mL Hex, 0.25 mL EtAc. The aqueous phase (sample) was completed with ultrapure water when needed, resulting in Hex:EtAc:ACN:Aqueous (3:1:3:2, V:V:V:V). Tissue samples were first vortexed for 30 min at 4oC with glass beads, and only then centrifuged. The upper phase was collected into a new tube with a Hamilton glass syringe, which was washed three times in solvent between samples. Hex was added (half the volume of the first extraction) to the 2 remaining phases for re-extraction. Samples were again vortexed and centrifuged, and upper and middle phases were collected separately. To reduce phospholipid loss, a re-extraction of middle phase was done with ACN:EtAc (3:1, V:V) (half the volume of first extraction). Extraction (solvents and water) and experiment blanks (PBS) were included. All extraction solvents had 50 μg/mL BHT. Extracted samples were kept dried at -20°C under Ar.

Sampleprep ID:

SP004598

Sampleprep Summary:

To extract lipids from tissue samples, a 3-phase liquid extraction was performed to separate neutral and polar lipids. First, tissue samples were measured on an analytical scale then transferred into glass tubes, containing already the internal standard mix (Splash) and TG 6:0/6:0/6:0. Then, to reduce sample loss, 0.75 mL of ACN were added to microtubes, vortexed, and transferred into the corresponding glass tubes. The remaining solvents were added to each sample: 0.75 mL Hex, 0.25 mL EtAc. The aqueous phase (sample) was completed with ultrapure water when needed, resulting in Hex:EtAc:ACN:Aqueous (3:1:3:2, V:V:V:V). Tissue samples were first vortexed for 30 min at 4oC with glass beads, and only then centrifuged. The upper phase was collected into a new tube with a Hamilton glass syringe, which was washed three times in solvent between samples. Hex was added (half the volume of the first extraction) to the 2 remaining phases for re-extraction. Samples were again vortexed and centrifuged, and upper and middle phases were collected separately. To reduce phospholipid loss, a re-extraction of middle phase was done with ACN:EtAc (3:1, V:V) (half the volume of first extraction). Extraction (solvents and water) and experiment blanks (PBS) were included. All extraction solvents had 50 μg/mL BHT. Extracted samples were kept dried at -20°C under Ar.

Combined analysis:

Analysis ID	AN007412
Chromatography ID	CH005616
MS ID	MS007104
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Thermo Dionex Ultimate 3000
Column	Thermo Accucore C18 (150 x 2.1 mm, 2.6 µm)
MS Type	ESI
MS instrument type	Orbitrap
MS instrument name	Thermo Q Exactive Orbitrap
Ion Mode	POSITIVE
Units	raw intensity

Chromatography:

Chromatography ID:	CH005616
Instrument Name:	Thermo Dionex Ultimate 3000
Column Name:	Thermo Accucore C18 (150 x 2.1 mm, 2.6 µm)
Column Temperature:	35°C
Flow Gradient:	0.0 min, 35% B; 4.0 min, 60% B; 8.0 min, 70% B; 16.0 min, 85% B; 25.0 min, 97% B.
Flow Rate:	400 µL/min
Solvent A:	50% Acetonitrile/50% Water; 10 mM ammonium formate; 0.1% formic acid
Solvent B:	88% Isopropanol/10% Acetonitrile/2% Water; 2 mM ammonium formate; 0.02 % formic acid
Chromatography Type:	Reversed phase

MS:

MS ID:	MS007104
Analysis ID:	AN007412
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	Orbitrap
MS Type:	ESI
MS Comments:	Data acquisition was conducted in positive ionization mode using Xcalibur software (v4.1.31.9). Full scan MS spectra were acquired in data-dependent MS2 (dd-MS2) mode at a resolution of 70 000 (at m/z 200) over a mass range of m/z 250-1200. The automatic gain control (AGC) target was set to 1 × 10⁶ with a maximum injection time of 100 ms. The 15 most intense precursor ions per sac cycle were selected for fragmentation. Precursor isolation was performed with a 1.0 m/z isolation window, and fragmentation was achieved using higher-energy collisional dissociation (HCD) at normalized collision energy (NCE) of 25 and 30 eV. MS/MS spectra were acquired in the ion trap at a resolution of 35,000 (at m/z 200) with an AGC target of 1 × 10⁵ and a maximum injection time of 80 ms.
Ion Mode:	POSITIVE