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MGP002644

UniProt Annotations

Entry Information
Gene Namereplication protein A2, 32kDa
Protein EntryRFA2_HUMAN
UniProt IDP15927
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=3; Name=1; IsoId=P15927-1; Sequence=Displayed; Name=2; IsoId=P15927-2; Sequence=VSP_017201; Note=No experimental confirmation available.; Name=3; IsoId=P15927-3; Sequence=VSP_017202; Note=No experimental confirmation available.;
FunctionAs part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. {ECO:0000269|PubMed:15205463, ECO:0000269|PubMed:17765923, ECO:0000269|PubMed:17959650, ECO:0000269|PubMed:19116208, ECO:0000269|PubMed:20154705, ECO:0000269|PubMed:21504906, ECO:0000269|PubMed:2406247, ECO:0000269|PubMed:24332808, ECO:0000269|PubMed:7697716, ECO:0000269|PubMed:7700386, ECO:0000269|PubMed:8702565, ECO:0000269|PubMed:9430682, ECO:0000269|PubMed:9765279}.
InductionTranslationally up-regulated in response to DNA damage (at protein level). {ECO:0000269|PubMed:23393223}.
InteractionO75419:CDC45; NbExp=4; IntAct=EBI-621404, EBI-374969; O60516:EIF4EBP3; NbExp=3; IntAct=EBI-621404, EBI-746950; P04406:GAPDH; NbExp=2; IntAct=EBI-621404, EBI-354056; P27694:RPA1; NbExp=4; IntAct=EBI-621404, EBI-621389; P35244:RPA3; NbExp=6; IntAct=EBI-621404, EBI-621428; Q9UJW9:SERTAD3; NbExp=5; IntAct=EBI-621404, EBI-748621; Q9NZC9:SMARCAL1; NbExp=12; IntAct=EBI-621404, EBI-5457961; Q9BVW5:TIPIN; NbExp=3; IntAct=EBI-621404, EBI-2515360; P13051:UNG; NbExp=6; IntAct=EBI-621404, EBI-1025947; P23025:XPA; NbExp=4; IntAct=EBI-621404, EBI-295222;
PtmDifferentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser- 4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence. {ECO:0000269|PubMed:12814551, ECO:0000269|PubMed:1318195, ECO:0000269|PubMed:20068231, ECO:0000269|PubMed:20154705, ECO:0000269|PubMed:21558276, ECO:0000269|PubMed:21731742, ECO:0000269|PubMed:8246944, ECO:0000269|PubMed:9139719, ECO:0000269|PubMed:9295339}.
PtmDNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. {ECO:0000269|PubMed:24332808}.
SimilarityBelongs to the replication factor A protein 2 family. {ECO:0000305}.
SimilarityContains 1 OB DNA-binding domain. {ECO:0000305}.
Subcellular LocationNucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.
SubunitComponent of the replication protein A complex (RPA/RP- A), a heterotrimeric complex composed of RPA1, RPA2 and RPA3. Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA). Interacts with SERTAD3. Interacts with TIPIN. Interacts with TIMELESS. Interacts with PPP4R2; the interaction is direct, DNA damage-dependent and mediates the recruitment of the PP4 catalytic subunit PPP4C. Interacts (hyperphosphorylated) with RAD51. Interacts with SMARCAL1; the interaction is direct and mediates the recruitment to the RPA complex of SMARCAL1. Interacts with RAD52 and XPA; those interactions are direct and associate RAD52 and XPA to the RPA complex. {ECO:0000269|PubMed:10449415, ECO:0000269|PubMed:10982866, ECO:0000269|PubMed:11081631, ECO:0000269|PubMed:17141802, ECO:0000269|PubMed:17296725, ECO:0000269|PubMed:17765923, ECO:0000269|PubMed:19793861, ECO:0000269|PubMed:19793862, ECO:0000269|PubMed:19793863, ECO:0000269|PubMed:20154705, ECO:0000269|PubMed:21504906, ECO:0000269|PubMed:21558276, ECO:0000269|PubMed:2406247, ECO:0000269|PubMed:24332808, ECO:0000269|PubMed:7700386, ECO:0000269|PubMed:8702565}.
Web ResourceName=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/RPA2ID42146ch1p35.html";
Web ResourceName=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/rpa2/";
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