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MGP003640

UniProt Annotations

Entry Information

Gene Name	eukaryotic translation initiation factor 3, subunit F
Protein Entry	EIF3F_HUMAN
UniProt ID	O00303
Species	Human

Comments

Comment type	Description
Catalytic Activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). {ECO:0000269\|PubMed:21124883}.
Domain	The MPN domain mediates deubiquitinating activity.
Function	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. {ECO:0000255\|HAMAP- Rule:MF_03005, ECO:0000269\|PubMed:21124883}.
Function	Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. {ECO:0000269\|PubMed:21124883}.
Interaction	P21127:CDK11B; NbExp=3; IntAct=EBI-711990, EBI-1298; P55884:EIF3B; NbExp=3; IntAct=EBI-711990, EBI-366696; Q969P5:FBXO32; NbExp=7; IntAct=EBI-711990, EBI-2932534; Q53GS7:GLE1; NbExp=2; IntAct=EBI-711990, EBI-1955541; O15457:MSH4; NbExp=6; IntAct=EBI-711990, EBI-6092777; Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-711990, EBI-6248094;
Mass Spectrometry	Mass=37475.6; Mass_error=0.2; Method=MALDI; Range=1-357; Evidence={ECO:0000269\|PubMed:18599441};
Mass Spectrometry	Mass=37554.8; Method=Unknown; Range=1-357; Evidence={ECO:0000269\|PubMed:17322308};
Ptm	Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. {ECO:0000269\|PubMed:12446680, ECO:0000269\|PubMed:17322308, ECO:0000269\|PubMed:18669648}.
Similarity	Belongs to the eIF-3 subunit F family. {ECO:0000255\|HAMAP-Rule:MF_03005}.
Similarity	Contains 1 MPN (JAB/Mov34) domain. {ECO:0000255\|HAMAP- Rule:MF_03005}.
Subcellular Location	Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_03005}.
Subunit	Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination- dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1. {ECO:0000255\|HAMAP- Rule:MF_03005, ECO:0000269\|PubMed:16286006, ECO:0000269\|PubMed:16766523, ECO:0000269\|PubMed:17322308, ECO:0000269\|PubMed:18599441, ECO:0000269\|PubMed:20068067, ECO:0000269\|PubMed:21124883}.
Web Resource	Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/EIF3FID44407ch11p15.html";