MGP Database

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MGP004057

UniProt Annotations

Entry Information
Gene NameRNA binding motif protein 8A
Protein EntryRBM8A_HUMAN
UniProt IDQ9Y5S9
SpeciesHuman
Comments
Comment typeDescription
Alternative ProductsEvent=Alternative splicing; Named isoforms=2; Name=1; Synonyms=BOV-1a; IsoId=Q9Y5S9-1; Sequence=Displayed; Name=2; Synonyms=BOV-1b; IsoId=Q9Y5S9-2; Sequence=VSP_005810;
FunctionCore component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator WIBG/PYM leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. {ECO:0000269|PubMed:12121612, ECO:0000269|PubMed:12718880, ECO:0000269|PubMed:12730685, ECO:0000269|PubMed:16209946, ECO:0000269|PubMed:19409878, ECO:0000269|PubMed:22203037}.
InteractionP38919:EIF4A3; NbExp=21; IntAct=EBI-447231, EBI-299104; P61326:MAGOH; NbExp=22; IntAct=EBI-447231, EBI-299134; Q96SB4:SRPK1; NbExp=2; IntAct=EBI-447231, EBI-539478; P78362:SRPK2; NbExp=2; IntAct=EBI-447231, EBI-593303; Q9H1J1:UPF3A; NbExp=4; IntAct=EBI-447231, EBI-521530; Q9BZI7:UPF3B; NbExp=9; IntAct=EBI-447231, EBI-372780;
Sequence CautionSequence=AAG14951.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAG16782.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAG16782.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA. A chimeric cDNA originating from chromosomes 1 and 5.; Evidence={ECO:0000305};
SimilarityBelongs to the RBM8A family. {ECO:0000305}.
SimilarityContains 1 RRM (RNA recognition motif) domain. {ECO:0000255|PROSITE-ProRule:PRU00176}.
Subcellular LocationNucleus {ECO:0000269|PubMed:19324961}. Nucleus speckle {ECO:0000269|PubMed:19324961}. Cytoplasm {ECO:0000269|PubMed:19324961}. Note=Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and UAP56 in the nucleus and nuclear speckles.
SubunitHeterodimer with RBM8A. Part of the mRNA splicing- dependent exon junction complex (EJC) complex; the core complex contains CASC3, EIF4A3, MAGOH and RBM8A. Interacts with WIBG/PYM; the interaction is direct and dissociates the EJC from spliced mRNAs. Found in a post-splicing complex with NXF1, RBM8A, UPF1, UPF2, UPF3A, UPF3B and RNPS1. Interacts with BAT1, MAGOH, OVCA1, UPF3B, RNPS1, SRRM1 and ALYREF/THOC4. Interacts with IPO13; the interaction mediates the nuclear import of the MAGOH-RBM8A heterodimer. Identified in the spliceosome C complex. Associates with polysomes. {ECO:0000269|PubMed:10662555, ECO:0000269|PubMed:11013075, ECO:0000269|PubMed:11118221, ECO:0000269|PubMed:11447110, ECO:0000269|PubMed:11546873, ECO:0000269|PubMed:11546874, ECO:0000269|PubMed:11707413, ECO:0000269|PubMed:11991638, ECO:0000269|PubMed:12718880, ECO:0000269|PubMed:12730685, ECO:0000269|PubMed:12781131, ECO:0000269|PubMed:12944400, ECO:0000269|PubMed:14625303, ECO:0000269|PubMed:14968132, ECO:0000269|PubMed:16170325, ECO:0000269|PubMed:16314458, ECO:0000269|PubMed:18026120, ECO:0000269|PubMed:19410547}.
Tissue SpecificityUbiquitous.
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