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MGP Database

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MGP005427

UniProt Annotations

Entry Information

Gene Name	phosphatidylinositol 4-kinase type 2 alpha
Protein Entry	P4K2A_HUMAN
UniProt ID	Q9BTU6
Species	Human

Comments

Comment type	Description
Catalytic Activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate. {ECO:0000269\|PubMed:11279162, ECO:0000269\|PubMed:20388919, ECO:0000269\|PubMed:24675427, ECO:0000269\|PubMed:25168678}.
Function	Membrane-bound phosphatidylinositol-4 kinase (PI4- kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). {ECO:0000269\|PubMed:11279162, ECO:0000269\|PubMed:16443754, ECO:0000269\|PubMed:20388919, ECO:0000269\|PubMed:23146885, ECO:0000269\|PubMed:24675427, ECO:0000269\|PubMed:25168678, ECO:0000305}.
Interaction	Q96J02:ITCH; NbExp=5; IntAct=EBI-3239392, EBI-1564678;
Ptm	Palmitoylated by ZDHHC3 and ZDHHC7 in the CCPCC motif. Palmitoylation is cholesterol-dependent, and required for TGN localization. {ECO:0000269\|PubMed:22535966}.
Ptm	Ubiquitinated by ITCH; this does not lead to proteasomal degradation. {ECO:0000269\|PubMed:23146885}.
Similarity	Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily. {ECO:0000305}.
Similarity	Contains 1 PI3K/PI4K domain. {ECO:0000305}.
Subcellular Location	Golgi apparatus, trans-Golgi network membrane {ECO:0000269\|PubMed:16443754, ECO:0000269\|PubMed:19690332, ECO:0000269\|PubMed:22535966}; Lipid- anchor {ECO:0000269\|PubMed:22535966}. Membrane raft {ECO:0000269\|PubMed:11279162}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q2TBE6}. Cell junction, synapse, presynaptic cell membrane {ECO:0000250\|UniProtKB:Q2TBE6}. Cell junction, synapse, synaptosome {ECO:0000250\|UniProtKB:Q2TBE6}. Mitochondrion {ECO:0000250\|UniProtKB:Q2TBE6}. Endosome {ECO:0000269\|PubMed:16443754, ECO:0000269\|PubMed:23146885}. Cytoplasmic vesicle {ECO:0000269\|PubMed:16443754, ECO:0000269\|PubMed:19690332}. Membrane {ECO:0000269\|PubMed:24675427}; Lipid-anchor {ECO:0000269\|PubMed:24675427}. Cell membrane {ECO:0000269\|PubMed:11279162, ECO:0000269\|PubMed:16443754}. Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP-3-complexes- dependent manner. Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity). Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. {ECO:0000250\|UniProtKB:Q2TBE6, ECO:0000250\|UniProtKB:Q99M64}.
Subunit	Associates with the BLOC-1 and the AP-3 complexes; the BLOC-1 complex is required for optimal binding of PI4K2A to the AP-3 complex. Interacts with BLOC1S5 and DTNBP1 (PubMed:21998198). Interacts with FOS; this interaction may enhance phosphatidylinositol phosphorylation activity (By similarity). Interacts with ITCH (PubMed:23146885). {ECO:0000250\|UniProtKB:Q2TBE6, ECO:0000269\|PubMed:21998198, ECO:0000269\|PubMed:23146885}.
Tissue Specificity	Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine. {ECO:0000269\|PubMed:11279162}.