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MB Sample ID: SA003963
Local Sample ID: | 13C/15N-Gln-plate-1-cells |
Subject ID: | SU000095 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | A549 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000095 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | A549 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
13C/15N-Gln-plate-1-cells | SA003963 | FL001022 | 13C/15N-Gln | Labeling |
Collection:
Collection ID: | CO000078 |
Collection Summary: | cell_quench_collection |
Collection Protocol Filename: | 14Jun17_Fan_Quench_cell_tissue_SOP.pdf |
Collection Protocol Comments: | Collection and quench of cells in tissue culture |
Sample Type: | Lung |
Treatment:
Treatment ID: | TR000096 |
Treatment Summary: | 13C-Glc|13C/15N-Gln|unlabeled |
Treatment Protocol Filename: | 14Jun17Fan_Cell_Tracer_Ex_SOP.pdf |
Treatment Protocol Comments: | Cell culture in 13C-Glc-labeled media | 13C/15N-Gln-labeled media | unlabeled media for 24h |
Sample Preparation:
Sampleprep ID: | SP000091 |
Sampleprep Summary: | protein_extraction|polar_preparation|polar_extraction |
Sampleprep Protocol Filename: | 14June20Fan_Prot_Quant_SOP.pdf 14Jun30Fan_Prep_Polar_SOP.pdf 14Jun30Fan_Extract_Polar_Lipid_Prot_SOP.pdf |
Sampleprep Protocol Comments: | Protein extraction and quantification|Preparation of polar samples for GCMS and NMR|Polar extraction from cells. |
Combined analysis:
Analysis ID | AN000121 | AN000122 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | GC | GC |
Chromatography system | ||
Column | ||
MS Type | ESI | EI |
MS instrument type | Single quadrupole | GC-ITQ |
MS instrument name | Thermo LTQ-FT | Thermo ITQ |
Ion Mode | NEGATIVE | POSITIVE |
Units | normalized corrected Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000083 |
Chromatography Type: | GC |
MS:
MS ID: | MS000097 |
Analysis ID: | AN000121 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formula |
Ion Mode: | NEGATIVE |
MS ID: | MS000098 |
Analysis ID: | AN000122 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
MS Comments: | GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivatization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide) |
Ion Mode: | POSITIVE |