Return to study ST000114 main page
MB Sample ID: SA006094
Local Sample ID: | tet-normox-13C-Glc-plate-2 |
Subject ID: | SU000133 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | P493 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000133 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | P493 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
tet-normox-13C-Glc-plate-2 | SA006094 | FL001577 | normoxia | Oxygen Condition |
tet-normox-13C-Glc-plate-2 | SA006094 | FL001577 | Tetracycline | Treatment |
Collection:
Collection ID: | CO000117 |
Collection Summary: | - |
Collection Protocol Comments: | Collection and quench of cells in tissue culture |
Sample Type: | B Cells |
Treatment:
Treatment ID: | TR000135 |
Treatment Protocol Comments: | tet-hypox-13C-Glc: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C]-Glc | tet-normox-13C-Glc: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C]-Glc | hypox-13C-Glc: cell culture under hypoxia for 24hr + 24hr with [U-13C]-Glc | normox-13C-Glc: cell culture under normoxia for 24hr + 24hr with [U-13C]-Glc |
Sample Preparation:
Sampleprep ID: | SP000130 |
Sampleprep Summary: | - |
Sampleprep Protocol Comments: | Lipid extraction and preparation for FTMS. | Protein extraction and quantification. | Preparation of polar samples for GCMS and NMR. |
Combined analysis:
Analysis ID | AN000192 | AN000193 | AN000194 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | GC | GC | GC |
Chromatography system | |||
Column | |||
MS Type | ESI | ESI | EI |
MS instrument type | Single quadrupole | Single quadrupole | GC-ITQ |
MS instrument name | Thermo LTQ-FT | Thermo LTQ-FT | Thermo ITQ |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
Units | Peak area | Peak area | normalized Peak area |
Chromatography:
Chromatography ID: | CH000128 |
Chromatography Type: | GC |
MS:
MS ID: | MS000155 |
Analysis ID: | AN000192 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000156 |
Analysis ID: | AN000193 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
MS ID: | MS000157 |
Analysis ID: | AN000194 |
Instrument Name: | Thermo ITQ |
Instrument Type: | GC-ITQ |
MS Type: | EI |
Ion Mode: | POSITIVE |