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MB Sample ID: SA006095
| Local Sample ID: | tet-normox-13C-Glc-plate-1 |
| Subject ID: | SU000133 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | P493 |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000133 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | P493 |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| tet-normox-13C-Glc-plate-1 | SA006095 | FL001577 | normoxia | Oxygen Condition |
| tet-normox-13C-Glc-plate-1 | SA006095 | FL001577 | Tetracycline | Treatment |
Collection:
| Collection ID: | CO000117 |
| Collection Summary: | - |
| Collection Protocol Comments: | Collection and quench of cells in tissue culture |
| Sample Type: | B Cells |
Treatment:
| Treatment ID: | TR000135 |
| Treatment Protocol Comments: | tet-hypox-13C-Glc: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C]-Glc | tet-normox-13C-Glc: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C]-Glc | hypox-13C-Glc: cell culture under hypoxia for 24hr + 24hr with [U-13C]-Glc | normox-13C-Glc: cell culture under normoxia for 24hr + 24hr with [U-13C]-Glc |
Sample Preparation:
| Sampleprep ID: | SP000130 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Comments: | Lipid extraction and preparation for FTMS. | Protein extraction and quantification. | Preparation of polar samples for GCMS and NMR. |
Combined analysis:
| Analysis ID | AN000192 | AN000193 | AN000194 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | GC | GC |
| Chromatography system | |||
| Column | |||
| MS Type | ESI | ESI | EI |
| MS instrument type | Single quadrupole | Single quadrupole | GC-ITQ |
| MS instrument name | Thermo LTQ-FT | Thermo LTQ-FT | Thermo ITQ |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
| Units | Peak area | Peak area | normalized Peak area |
Chromatography:
| Chromatography ID: | CH000128 |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000155 |
| Analysis ID: | AN000192 |
| Instrument Name: | Thermo LTQ-FT |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| MS ID: | MS000156 |
| Analysis ID: | AN000193 |
| Instrument Name: | Thermo LTQ-FT |
| Instrument Type: | Single quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000157 |
| Analysis ID: | AN000194 |
| Instrument Name: | Thermo ITQ |
| Instrument Type: | GC-ITQ |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
