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MB Sample ID: SA012095
| Local Sample ID: | QE1_REP_18_10 |
| Subject ID: | SU000289 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 8 or 24 week |
| Gender: | male |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000289 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 8 or 24 week |
| Gender: | male |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| QE1_REP_18_10 | SA012095 | FL003018 | 24 | Age (weeks) |
| QE1_REP_18_10 | SA012095 | FL003018 | Trans Fat Diet | Diet |
Collection:
| Collection ID: | CO000283 |
| Collection Summary: | - |
| Sample Type: | tissue (liver) |
| Collection Method: | tissue flash frozen in liquid nitrogen immediately after sacrifice |
Treatment:
| Treatment ID: | TR000303 |
| Treatment Summary: | Mice were either fed a control diet or high fructose trans-fat diet for 8 or 24 weeks |
| Treatment Compound: | diet (Research Diets, Inc) |
| Treatment Route: | oral |
| Animal Anesthesia: | isoflurane (3% in O2) |
| Animal Endp Clinical Signs: | accompanied histology |
Sample Preparation:
| Sampleprep ID: | SP000297 |
| Sampleprep Summary: | Tissues were cryogenically ground prior to beign weighed and homogenized in chloroform and methanol for the Folch extraction |
| Processing Method: | homogenization (zirconium beads, 1.0 mm diameter), lipid extraction |
| Processing Storage Conditions: | Liquid nitrogen and on ice |
| Extraction Method: | Folch |
| Extract Storage: | -80C |
| Sample Resuspension: | isopropanol |
| Sample Spiking: | Internal Standards: Cer(d18:1/17:0) and d5-DAG (20:2/20:2) and d5-DG (20:5/20:5) |
| Organ: | liver |
Combined analysis:
| Analysis ID | AN000431 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | nmol/g liver protein |
Chromatography:
| Chromatography ID: | CH000301 |
| Chromatography Summary: | Waters Acquity BEH C18 (2.1 x 50 mm, 1.7 um) with guard was used to separate compounds with mobile phases made of isopropanol, acetonitrile, and water. |
| Methods Filename: | REP_18 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 30 C |
| Flow Gradient: | 32% B to 98% B over 18 mins |
| Flow Rate: | 0.5 mL/min |
| Injection Temperature: | 4 C |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 18 mins |
| Capillary Voltage: | 350 C |
| Time Program: | 0-1 min: 32%B --> 1-1.5 min: 40% B --> 4 min: 45% B --> 5 min: 50% B --> 8 min: 60% B --> 11 min: 70% B --> 14 min: 80% B --> 16-17 min: 100%B --> 18 min: 32%B --> 21 min: 32% B |
| Strong Wash Solvent Name: | 1:1:1 isopropanol:acetonitrile:water |
| Strong Wash Volume: | 100 uL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000372 |
| Analysis ID: | AN000431 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 300 |
| Dry Gas Flow: | 30 |
| Ion Source Temperature: | 350 |
| Ion Spray Voltage: | 3500 |
| Mass Accuracy: | 3 ppm |
| Automatic Gain Control: | 3000000 |
| Desolvation Temperature: | 350 |
| Resolution Setting: | 17500 |
| Scan Range Moverz: | 150-2000 |
| Acquisition Parameters File: | REP_18 |
| Processing Parameters File: | REP_18_sequence_processing |
