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MB Sample ID: SA030817
Local Sample ID: | G9_6 |
Subject ID: | SU000606 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | Contains oral bacteria species |
Age Or Age Range: | 18-65 years |
Gender: | F/M |
Human Race: | Various |
Human Ethnicity: | Various |
Human Lifestyle Factors: | Various |
Human Medications: | None |
Human Prescription Otc: | None |
Human Smoking Status: | None |
Human Alcohol Drug Use: | None |
Human Nutrition: | Various |
Human Inclusion Criteria: | Age 18 to 65 years, caries-free or caries-actie, no systemic disease |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000606 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | Contains oral bacteria species |
Age Or Age Range: | 18-65 years |
Gender: | F/M |
Human Race: | Various |
Human Ethnicity: | Various |
Human Lifestyle Factors: | Various |
Human Medications: | None |
Human Prescription Otc: | None |
Human Smoking Status: | None |
Human Alcohol Drug Use: | None |
Human Nutrition: | Various |
Human Inclusion Criteria: | Age 18 to 65 years, caries-free or caries-actie, no systemic disease |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
G9_6 | SA030817 | FL007164 | caries-active | caries status |
G9_6 | SA030817 | FL007164 | baseline | time |
Collection:
Collection ID: | CO000600 |
Collection Summary: | Supragingival plaque samples were collected from specific tooth sites using sterile dental curetes. |
Sample Type: | Plaque samples |
Collection Method: | Scrapping of tooth surfaces |
Collection Location: | Multiple teeth |
Collection Frequency: | Once |
Collection Duration: | 5 min |
Volumeoramount Collected: | 250ul |
Storage Conditions: | freezer at -80 |
Collection Vials: | eppendorfs containing phosphate buffer pH 7.0 |
Storage Vials: | eppendorfs containing phosphate buffer pH 7.0 |
Collection Tube Temp: | 4 |
Treatment:
Treatment ID: | TR000620 |
Treatment Summary: | Subjects made use for 12 weeks of either a fluoride-free 1.5% arginine toothpaste or a fluoride containing toothpaste (1100 ppm F as NaF). |
Sample Preparation:
Sampleprep ID: | SP000613 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Combined analysis:
Analysis ID | AN000894 | AN000895 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | unspecified | unspecified |
Chromatography:
Chromatography ID: | CH000637 |
Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000796 |
Analysis ID: | AN000894 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
MS ID: | MS000797 |
Analysis ID: | AN000895 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |