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MB Sample ID: SA046884
| Local Sample ID: | PLAZ-13 |
| Subject ID: | SU000871 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000871 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PLAZ-13 | SA046884 | FL009263 | Simvastatin | Treatment |
| PLAZ-13 | SA046884 | FL009263 | Day 8 | Day |
Collection:
| Collection ID: | CO000865 |
| Collection Summary: | Monkeys were treated with placebo or Provastatin for 12 days. Further, after the wash out period animals were treated with Simvastatin for 12 days. Lung wash fluid was collected at day 0, 8 and 12 of each treatment. |
| Sample Type: | Blood |
| Blood Serum Or Plasma: | Plasma |
Treatment:
| Treatment ID: | TR000885 |
| Treatment Summary: | Monkeys were treated (by inhalation) with placebo or Provastatin for 12 days. Further, after the wash out period animals were treated with Simvastatin for 12 days. Lung wash fluid was collected at day 0, 8 and 12 of each treatment. |
Sample Preparation:
| Sampleprep ID: | SP000878 |
| Sampleprep Summary: | Oxylipins, endocannabinoids, bile acids and fatty acids were isolated from an aliquot of 20μl plasma in Eppendorf tubes. The tube was spiked was 5ul of OxyEndo Fusion, 10μl spike of Bile Acid SSTD Solutions, and 5μl of BHT/EDTA solution. A total of 100μl CUDA/PHAU in methanol was added to the tube, before it was capped, vortexed, and centrifuged (3 min, 10,000 RCF, room temp). It was then placed on wet ice for 5 min. Next it was spin filtered with Millapore spin filters (0.1 µm, Millipore, Billerica, MA) at 8 ºC, 4500 RPM for 3 min, before being transferred to 2 mL LC-MS amber vials. Extracts were stored at -20ºC until analysis by UPLC-MS/MS. The internal standard was used to quantify the recovery of surrogate standards. |
Combined analysis:
| Analysis ID | AN001366 | AN001367 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Concentration (nM) | Concentration (nM) |
Chromatography:
| Chromatography ID: | CH000953 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
| Column Temperature: | 60 °C |
| Flow Gradient: | See protocol/methods file |
| Flow Rate: | 0.25 mL/min |
| Internal Standard: | See protocol/methods file |
| Retention Time: | See protocol/methods file |
| Sample Injection: | 5 µL |
| Solvent A: | 100% water; 0.1% acetic acid |
| Solvent B: | 90% acetonitrile/ 10% isopropanol |
| Analytical Time: | 20 min |
| Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
| Weak Wash Volume: | 600 µL |
| Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
| Strong Wash Volume: | 600 µL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001258 |
| Analysis ID: | AN001366 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| MS ID: | MS001259 |
| Analysis ID: | AN001367 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
