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MB Sample ID: SA074765
Local Sample ID: | S23_1 |
Subject ID: | SU001140 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
Genotype Strain: | Col-0/Cvi-0/Bur-0/MIB-22/Shahdara/Fei-0/Bor-4/Ts-1/Tsu-0/RRS-7/Est-1/Ler-1/C24/RRS-10/Bla-1/Br-0/Got-7/Kas-2/Lov-5/Mr-0/Pna-17/T540/Tamm-2/UKNW06-460/Ty-0 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001140 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
Genotype Strain: | Col-0/Cvi-0/Bur-0/MIB-22/Shahdara/Fei-0/Bor-4/Ts-1/Tsu-0/RRS-7/Est-1/Ler-1/C24/RRS-10/Bla-1/Br-0/Got-7/Kas-2/Lov-5/Mr-0/Pna-17/T540/Tamm-2/UKNW06-460/Ty-0 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S23_1 | SA074765 | FL011563 | UKNW06-460 | ecotype |
S23_1 | SA074765 | FL011563 | shoot | tissue |
Collection:
Collection ID: | CO001134 |
Collection Summary: | The ABRC stock IDs are stored in the uploaded data folder. Sterilized seeds were kept for 2 days at 4 degreeC in the dark, and then sown on Murashige and Skoog (MS) medium agar containing 1% sucrose. All plates were placed in a growth chamber at 22 degreeC under a 16-hr light (approximately 40 micro mol/(sec m^2)/8-hr dark cycle. After 3-week cultivation the plants were harvested and shoot and root parts were immediately frozen in liquid nitrogen and lyophilized at -55 degreeC. Lyophilized samples were stored at -80 degreeC until use. Three technical replicates for each strain were prepared from quenched samples. |
Sample Type: | Plant |
Treatment:
Treatment ID: | TR001154 |
Treatment Summary: | NA |
Sample Preparation:
Sampleprep ID: | SP001147 |
Sampleprep Summary: | Samples (3 mg) of dried plant tissues were weighted in a 2.0-ml microtube (Sarstedt). Their metabolites were extracted with 150 µl of 80% MeOH containing 2.5 µM lidocaine and 10-camphour sulfonic acid per mg of dry weight using a mixer-mill with zirconia beads [7 min at 18 Hz and 4 degree C (MM300; Retsch)]. After 10-min centrifugation at 17,800 g the supernatant was filtered using an HLB µElution plate (Waters). |
Combined analysis:
Analysis ID | AN001783 | AN001784 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 S QTOF | Waters Synapt G2 S QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak intensity | Peak intensity |
Chromatography:
Chromatography ID: | CH001260 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 40 |
Flow Gradient: | 99.5% solvent A/0.5% solvent B at 0 min, 99.5%A/0.5%B at 0.1 min, 20%A/80%B at 10 min, 0.5%A/99.5%B at 10.1 min, 0.5%A/99.5%B at 12.0 min, 99.5%A/0.5%B at 12.1 min and 99.5%A/0.5%B at 15.0 min |
Flow Rate: | 0.3 ml/min at 0 min, 0.3 ml/min at 10 min, 0.4 ml/min at 10.1 min, 0.4 ml/min at 14.4 min, and 0.3 ml/min at 14.5 min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001647 |
Analysis ID: | AN001783 |
Instrument Name: | Waters Synapt G2 S QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | +3.00 kV |
Collision Energy: | 6 V |
Source Temperature: | 120 |
Desolvation Gas Flow: | 800 l/hr |
Desolvation Temperature: | 450 degree C |
Scan Range Moverz: | 50 - 1500 |
MS ID: | MS001648 |
Analysis ID: | AN001784 |
Instrument Name: | Waters Synapt G2 S QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Voltage: | -2.75 kV |
Collision Energy: | 6 V |
Source Temperature: | 120 |
Desolvation Gas Flow: | 800 l/hr |
Desolvation Temperature: | 450 degree C |
Scan Range Moverz: | 50 - 1500 |