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MB Sample ID: SA077748
| Local Sample ID: | S+_02 |
| Subject ID: | SU001177 |
| Subject Type: | Cell line |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001177 |
| Subject Type: | Cell line |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S+_02 | SA077748 | FL011824 | T24S | Cell line |
| S+_02 | SA077748 | FL011824 | Yes | Treatment |
Collection:
| Collection ID: | CO001171 |
| Collection Summary: | T24 BC cells were obtained and cultured, according to instructions provided by the ATCC. Media was supplemented with 10% fetal bovine serum, 2% glutamine and 1% antibiotics (Invitrogen, Carlsbad, CA). Cells were maintained under a humidified atmosphere of 5% CO2 at 37°C. These cell lines were negative for mycoplasma and all other infectious agents evaluated. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR001192 |
| Treatment Summary: | T24 BC cells were characterized in our laboratory as cisplatin resistant BC (T24R, R) or cisplatin sensitive BC cells (T24S, S) to cisplatin-induced apoptosis. |
Sample Preparation:
| Sampleprep ID: | SP001185 |
| Sampleprep Summary: | We separated lipid extracts from proteins and polar hydrophilic small molecules in a way where the lipids are found in the top layer of liquid-liquid separations, rather than in the bottom layer. Decanting the top layer, therefore, ensured that proteins or polar compounds did not contaminate extracts. |
Combined analysis:
| Analysis ID | AN001832 | AN001833 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | normalized_intensity | normalized_intensity |
Chromatography:
| Chromatography ID: | CH001311 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001693 |
| Analysis ID: | AN001832 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | POSITIVE |
| MS ID: | MS001694 |
| Analysis ID: | AN001833 |
| Instrument Name: | Agilent 6550 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | none |
| Ion Mode: | NEGATIVE |
