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MB Sample ID: SA080350
Local Sample ID: | JHOP-05743 |
Subject ID: | SU001223 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001223 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
JHOP-05743 | SA080350 | FL012312 | Feces | Sample |
JHOP-05743 | SA080350 | FL012312 | Angiotensin II | Treatment |
Collection:
Collection ID: | CO001217 |
Collection Summary: | Feces |
Collection Protocol Filename: | Sample_Collection-Feces_Pluznick.pdf |
Sample Type: | Feces |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001238 |
Treatment Summary: | Six week old C57BL/6 conventional (mice with gut microbiota; conv, n=6) and germ-free (mice without gut microbiota; GF, n=6) mice were infused with angiotensin-II (AngII) for 4 weeks (400ng.kg-1.min-1; Alzet 1004). In parallel control groups, conv (n=6) and GF (n=6) mice received saline via minipumps. |
Treatment Protocol Filename: | Treatment_Protocol__Pluznick.pdf |
Sample Preparation:
Sampleprep ID: | SP001231 |
Sampleprep Summary: | Samples were prepared using the automated MicroLab STAR® system from Hamilton Company. Several recovery standards were added prior to the first step in the extraction process for QC purposes. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP)/UPLC-MS/MS methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by HILIC/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup. Samples were placed briefly on a TurboVap® (Zymark) to remove the organic solvent. The sample extracts were stored overnight under nitrogen before preparation for analysis. |
Sampleprep Protocol Filename: | Sample_Preparation_Pluznick.pdf |
Combined analysis:
Analysis ID | AN001916 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer |
Column | C18 |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE |
Units | Peaks area |
Chromatography:
Chromatography ID: | CH001389 |
Methods Filename: | Ultrahigh_Performance_LC_Method_Pluznick.pdf |
Instrument Name: | Thermo Scientific Q-Exactive high resolution/accurate mass spectrometer |
Column Name: | C18 |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001772 |
Analysis ID: | AN001916 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | None |
Ion Mode: | NEGATIVE |