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MB Sample ID: SA093866
Local Sample ID: | 82632 |
Subject ID: | SU001364 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001364 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
82632 | SA093866 | FL013433 | RML | Inocula |
82632 | SA093866 | FL013433 | 90 dpi | Timepoint |
Collection:
Collection ID: | CO001359 |
Collection Summary: | Brains were harvested and fash-frozen with dry ice |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR001379 |
Treatment Summary: | Wild type FVB mice were subjected to intracerebral injection to be inoculated with 30ul of 1% (w.v) RML brain homogenate or normal brain homogenate. At 30, 60, 90, 120 and 150 dpi, a sub-set of RML and NBH inoculated animals were euthanized. |
Sample Preparation:
Sampleprep ID: | SP001372 |
Sampleprep Summary: | Half brains were homogenized with Dounce tissue homogenizers to make 10% (w/v) homogenate. The homogenate is then subject to ultracentrifuge at 100,000xg for 1h at 4°C, the resulting supernatant was neutralized with Tris buffer (0.5M, pH6.8) one tenth the volume of the supernatant. Then the neutralized supernatant is passed through 3kDa molecular weight cut-off filters at 4000 rpm for 45 mins at 4°C. Filtered supernatant was tried at SpeedVac Vacuum Concentrator at low heating until try pellets were received. Try pellets were then dissolved with 530ul of water and 60 ul of D2O containing 5mM of DSS. A final volume of 600ul for each sample was achieved. |
Analysis:
MB Sample ID: | SA093866 |
Analysis ID: | AN002146 |
Analysis Type: | NMR |
Num Factors: | 10 |
Num Metabolites: | 50 |
Units: | uM |
NMR:
NMR ID: | NM000156 |
Analysis ID: | AN002146 |
Instrument Name: | INOVA NMR spectrometer |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 500MHz |