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MB Sample ID: SA096791
| Local Sample ID: | day_5_Mutant_5_2 |
| Subject ID: | SU001408 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001408 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| day_5_Mutant_5_2 | SA096791 | FL013648 | Mutant | Animal |
| day_5_Mutant_5_2 | SA096791 | FL013648 | Day 5 irrad | Radiation Duration |
Collection:
| Collection ID: | CO001403 |
| Collection Summary: | At the time of sacrifice animals were transcardially perfused with 5 ml phosphate buffered saline (PBS) and ileum was snap frozen. |
| Sample Type: | Ileum |
Treatment:
| Treatment ID: | TR001423 |
| Treatment Summary: | Mice were anaesthetized with Nembutal (50 mg/kg) and irradiated to 9.25Gy using a Shepherd Mark 1 Model 68 cesium irradiator at a dose rate of 80 cGy/minute, as described (Huang et al., 2016). |
Sample Preparation:
| Sampleprep ID: | SP001416 |
| Sampleprep Summary: | Total Lipid Extraction. Lipids were extracted from using the Folch method (Folch et al., 1957). Total phospholipid content was quantified using the micro method for phosphorus measurement (Böttcher et al., 1961). Tissue CL and PE content was quantified as described previously (Wenzel et al., 2017). |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN002223 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Luna C18 (150 x 4.6mm,3um,100Å) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE |
| Units | Normalized amount |
Chromatography:
| Chromatography ID: | CH001631 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Luna C18 (150 x 4.6mm,3um,100Å) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002069 |
| Analysis ID: | AN002223 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Compound discoverer |
| Ion Mode: | NEGATIVE |
