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MB Sample ID: SA122108
Local Sample ID: | CSI-wt5 |
Subject ID: | SU001513 |
Subject Type: | Bacteria |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 weeks of age |
Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001513 |
Subject Type: | Bacteria |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 weeks of age |
Gender: | Male |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CSI-wt5 | SA122108 | FL014932 | wild type | Genotype |
Collection:
Collection ID: | CO001508 |
Collection Summary: | Mice were euthanized and collect half length of small intestine (ileum). Contents of small intestine were mechanically squeezed by pipet tip into 1.5 mL tube and then flash-frozen in liquid nitrogen. |
Sample Type: | contents of small intestine |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001528 |
Treatment Summary: | Mice were generated by in vitro fertilization and transplantation to surrogate mothers, and pups C-sectioned on E20 were fostered to CD-1 mother with strain-selected microbiota in the animal facility of CIEA. |
Sample Preparation:
Sampleprep ID: | SP001521 |
Sampleprep Summary: | For extracting ionic metabolites. approximately 20 mg of contents of small intestine were dissolved in H2O containing internal standards (H3304-1002, HMT, Japan) with ratio of 1:9. After centrifugation, the supernatant was the centrifugically filtered through a Milipore 5000-Da cutoff filter to remove macromolecules for subsequent analysis with CE-TOFMS. For extracting non-ionic metabolites, approximately 20 mg of contents of small intestine were dissolved in 1 mL of methanol containing internal standard (H3304-1002, HMT). After centrifugation, 1o uL of the supernatant was transferred into grass vials for evaporation under nitrogen gas and reconstituted with 200 uL of 50% isopropanol for subsequent analysis with LC-TOFMS. |
Combined analysis:
Analysis ID | AN002403 | AN002404 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | None (Direct infusion) | None (Direct infusion) |
Chromatography system | none | none |
Column | none | none |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6210 TOF | Agilent 6230 TOF |
Ion Mode | UNSPECIFIED | UNSPECIFIED |
Units | relative_area | relative_area |
Chromatography:
Chromatography ID: | CH001766 |
Instrument Name: | none |
Column Name: | none |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002244 |
Analysis ID: | AN002403 |
Instrument Name: | Agilent 6210 TOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | System control: Agilent G2201AA ChemStation software version B .03.01 Peaks were extracted using automatic integration software MasterHands to obtain peal information including m/z, peak area and migration time. Detected metabolites were plotted on metabolic pathway maps using VANTED software. |
Ion Mode: | UNSPECIFIED |
MS ID: | MS002245 |
Analysis ID: | AN002404 |
Instrument Name: | Agilent 6230 TOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | System control: Agilent G2201AA ChemStation software version B .03.01 Peaks were extracted using automatic integration software MasterHands to obtain peal information including m/z, peak area and retention time. Detected metabolites were plotted on metabolic pathway maps using VANTED software. |
Ion Mode: | UNSPECIFIED |