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MB Sample ID: SA154467
| Local Sample ID: | GOLPH3_KD_3 |
| Subject ID: | SU001750 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001750 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| GOLPH3_KD_3 | SA154467 | FL018157 | GOLPH3 silencing | Treatment |
Collection:
| Collection ID: | CO001743 |
| Collection Summary: | Cells were washed in ice cold PBS and collected by scaling |
| Sample Type: | HeLa cells |
Treatment:
| Treatment ID: | TR001763 |
| Treatment Summary: | Cells were treated to overexposes or down regulate GOLPH3 or LCS |
Sample Preparation:
| Sampleprep ID: | SP001756 |
| Sampleprep Summary: | Samples were fortified with an Internal standard Mix and lipids were extracted twice with an extraction mix consisting of 85:15 Ethyl acetate 70% Isopropanol. All lipids that were used in the Internal standard mix and in the Calibration mixes were purchased from Avanti Polar Lipids Inc. After evaporating the cell extract to dryness, the samples were reconstituted in the mobile phase. |
Combined analysis:
| Analysis ID | AN002731 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela 1250 |
| Column | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Quantum Ultra |
| Ion Mode | POSITIVE |
| Units | pmoles |
Chromatography:
| Chromatography ID: | CH002017 |
| Chromatography Summary: | Samples were analyzed with a Quantum Ultra triple quadrupole mass spectrometer connected to an Accela HPLC and Accela autosampler using a solvent gradient. Ceramides identity was achieved through MRM analysis with soft fragmentation. Quantitative analysis is based on calibration curves generated for each ceramide. J. Bielawski et al. / Methods 39 (2006) 82–91 |
| Instrument Name: | Thermo Accela 1250 |
| Column Name: | Thermo Accucore C18 (100 x 2.1mm,2.6um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002528 |
| Analysis ID: | AN002731 |
| Instrument Name: | Thermo Quantum Ultra |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Samples were analyzed with a Quantum Ultra triple quadrupole mass spectrometer connected to an Accela HPLC and Accela autosampler using a solvent gradient. Ceramides identity was achieved through MRM analysis with soft fragmentation. Quantitative analysis is based on calibration curves generated for each ceramide. J. Bielawski et al. / Methods 39 (2006) 82–91 |
| Ion Mode: | POSITIVE |
