Return to study ST001689 main page
MB Sample ID: SA156638
Local Sample ID: | 740 _1 |
Subject ID: | SU001766 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001766 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Not applicable |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
740 _1 | SA156638 | FL018360 | Delivery | Sample Code |
Collection:
Collection ID: | CO001759 |
Collection Summary: | Breast milk samples were collected at room temperature and stored at −80 °C until analyzed. |
Sample Type: | Breast milk |
Treatment:
Treatment ID: | TR001779 |
Treatment Summary: | Sample were stored at -80 as it is, no treatment done. |
Sample Preparation:
Sampleprep ID: | SP001772 |
Sampleprep Summary: | 30 µl breast milk sample or 10 µl of serum of was extracted with using a modified version of the previously published Folch procedure (Nygren et al. 2011). In short, 10 µL of 0.9% NaCl and, 120 µL of CHCl3: MeOH (2:1, v/v) containing the internal standards (c = 2.5 µg/mL) was added to the sample. The standard solution contained the following compounds: 1,2-diheptadecanoyl-sn-glycero-3-phosphoethanolamine (PE(17:0/17:0)), N-heptadecanoyl-D-erythro-sphingosylphosphorylcholine (SM(d18:1/17:0)), N-heptadecanoyl-D-erythro-sphingosine (Cer(d18:1/17:0)), 1,2-diheptadecanoyl-sn-glycero-3-phosphocholine (PC(17:0/17:0)), 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC(17:0)) and 1-palmitoyl-d31-2-oleoyl-sn-glycero-3-phosphocholine (PC(16:0/d31/18:1)), were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA), and, triheptadecanoylglycerol (TG(17:0/17:0/17:0)) was purchased from Larodan AB (Solna, Sweden). The samples were vortex mixed and incubated on ice for 30 min after which they were centrifuged (9400 × g, 3 min). 60 µL from the lower layer of each sample was then transferred to a glass vial with an insert and 60 µL of CHCl3: MeOH (2:1, v/v) was added to each sample. The samples were stored at -80 °C until analysis. |
Combined analysis:
Analysis ID | AN002759 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6210 TOF |
Ion Mode | NEGATIVE |
Units | ng/ml |
Chromatography:
Chromatography ID: | CH002039 |
Chromatography Summary: | BEH C18 (2.1 x 100 mm, particle size 1.7 µm) (Waters Corporation, Milford, MA, USA) |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002556 |
Analysis ID: | AN002759 |
Instrument Name: | Agilent 6210 TOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Dual jet stream electrospray (dual ESI) ion source was used and the ion polarity was on negative mode. The capillary voltage and the nozzle voltage were kept at 4500 V and 1500 V. The N2 pressure was set on 21 psi, with the sheath gas flow as 11 L/min and temperature at 379°C for the nebulizer. The data was acquired with MassHunter B.06.01 software (Agilent Technologies, Santa Clara, CA, The United States of America). |
Ion Mode: | NEGATIVE |