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MB Sample ID: SA252362
| Local Sample ID: | PLC-KO-4_POS |
| Subject ID: | SU002605 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002605 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| PLC-KO-4_POS | SA252362 | FL032077 | circLARP2-deficient | Genotype |
Collection:
| Collection ID: | CO002598 |
| Collection Summary: | Cells were counted, washed with cold PBS abd then flash-frozen in liquid N2 |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002617 |
| Treatment Summary: | Wild-type and circLARP2-deficient PLC cells with no Special treatment |
Sample Preparation:
| Sampleprep ID: | SP002611 |
| Sampleprep Summary: | The cells were washed with PBS under 37°C and the PBS was removed. 800 μL of cold methanol/acetonitrile (1:1, v/v) to remove the protein and extract the metabolites. The mixture was collected into a new centrifuge tube, and centrifuged at 14000g for 20 min to collect the supernatant. The supernatant was dried in a vacuum centri fuge. For LC MS analysis, the samples were redissolved in 100 μL acetonitrile/water (1:1, v/v) solvent and centrifuged at 14000 g at 4 ℃ for 15 min, then the supernatant was injected. |
Combined analysis:
| Analysis ID | AN004126 | AN004127 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 6600 TripleTOF | ABI Sciex 6600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH003057 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Column Temperature: | 25℃ |
| Flow Gradient: | The gradient was 95% B for 0.5 min and was linearly reduced to 65% in 6.5 min, and then was reduced to 40% in 1 min and kept for 1 min, and then increased to 95% in 0.1 min, with a 3 min |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate; 25 mM ammonium hydroxide |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003873 |
| Analysis ID: | AN004126 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | The mobile phase contained A=25 mM ammonium acetate and 25 mM ammonium hydroxide in water and B= acetonitrile. The gr adient was 95% B for 0.5 min and was linearly reduced to 65% in 6.5 min, and then was reduced to 40% in 1 min and kept for 1 min, and then increased to 95% in 0.1 min, with a 3 min reequilibration period employed. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003874 |
| Analysis ID: | AN004127 |
| Instrument Name: | ABI Sciex 6600 TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| MS Comments: | The mobile phase contained A=25 mM ammonium acetate and 25 mM ammonium hydroxide in water and B= acetonitrile. The gr adient was 95% B for 0.5 min and was linearly reduced to 65% in 6.5 min, and then was reduced to 40% in 1 min and kept for 1 min, and then increased to 95% in 0.1 min, with a 3 min reequilibration period employed. |
| Ion Mode: | NEGATIVE |
