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MB Sample ID: SA298607
| Local Sample ID: | HFD_CD_CD_802 |
| Subject ID: | SU002888 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6JRcc |
| Age Or Age Range: | 10-12 weeks |
| Animal Housing: | specific pathogen-free conditions |
| Animal Light Cycle: | 12-h light/dark cycle |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002888 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6JRcc |
| Age Or Age Range: | 10-12 weeks |
| Animal Housing: | specific pathogen-free conditions |
| Animal Light Cycle: | 12-h light/dark cycle |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| HFD_CD_CD_802 | SA298607 | FL036140 | HFD_CD_CD | diet |
Collection:
| Collection ID: | CO002881 |
| Collection Summary: | Livers were isolated from PBS-perfused mice, and 10 µg was homogenized in ddH2O using a Precellys homogenizer (Peqlab Biotechnology). The mice were subjected different diet schemes. |
| Collection Protocol Filename: | Lipidomics_Notes.pdf |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR002897 |
| Treatment Summary: | Samples were taken from the developed Maternal Obesity model. This model was generated as described in the Material and Methods section. No additional treatment was done. |
Sample Preparation:
| Sampleprep ID: | SP002894 |
| Sampleprep Summary: | In this experiment, mouse livers were isolated and processed to extract lipids for analysis. The livers were homogenized and subjected to a series of centrifugation and phase separation steps to obtain the lipid samples. The extracted lipids were then prepared for further analysis by adding specific internal standards and a spray buffer, allowing for subsequent characterization and investigation. |
| Sampleprep Protocol Filename: | Lipidomics_Notes.pdf |
Combined analysis:
| Analysis ID | AN004528 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | Thermo Q Exactive Plus |
| Column | none |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | pmol/mg |
Chromatography:
| Chromatography ID: | CH003401 |
| Instrument Name: | Thermo Q Exactive Plus |
| Column Name: | none |
| Column Temperature: | none |
| Flow Gradient: | - |
| Flow Rate: | 10 µl/min |
| Injection Temperature: | 260 |
| Solvent A: | 8/5/1 isopropanol/methanol/water; 10 mM ammonium acetate |
| Solvent B: | - |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS004275 |
| Analysis ID: | AN004528 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Thermo Q Exactive Plus spectrometer equipped with the HESI II ion source. MS1 spectra (resolution 280 000) were recorded in 100 m/z windows from 250 to 1200 m/z (pos.) and 200 - 1700 m/z (neg.) followed by recording MS/MS spectra (res. 70 000) by data-independent acquisition in 1 m/z windows from 200 to 1200 (pos.) and 200 to 1700 (neg.) m/z. Raw files were converted to .mzml files and imported into and analyzed by LipidXplorer software using custom mfql files to identify sample lipids and internal standards. For further data processing, absolute amounts were calculated using the internal standard intensities followed by the calculated mol% of the identified lipids. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Lipidomics_Notes.pdf |
