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MB Sample ID: SA318157
Local Sample ID: | bs_s4 |
Subject ID: | SU003044 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 20 wks |
Gender: | Male |
Animal Animal Supplier: | Jackson Lab |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003044 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 20 wks |
Gender: | Male |
Animal Animal Supplier: | Jackson Lab |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
bs_s4 | SA318157 | FL037864 | brainstem | Sample |
bs_s4 | SA318157 | FL037864 | Sedentary | Treatment |
Collection:
Collection ID: | CO003037 |
Collection Summary: | Mice were randomized into two groups, sedentary control and running experimental. Mice were housed with free access to a wireless running wheel (Med Associates). For the sedentary group, the wheels were locked in the experiment. After 42 days, blood (130 uL), feces, hippocampus and brainstem were collected and stored at -80°C. |
Sample Type: | blood, feces, hippocampus, brainstem |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003053 |
Treatment Summary: | To evaluate our hypothesis that running exercise reshapes gut microbiota diversity that balances TRP metabolism in the gut, we used an established model of voluntary running exercise in 20-week-old male mice (C57BL/6J, Jackson Lab) housed individually in a standard cage in temperature-controlled (21°C) quarters with a 12-h light/12-h dark cycle. Animals were given water and food (Purina Chow) ad libitum as previously described (Chorna et al., 2013). Briefly, mice were randomized into two groups, sedentary control (n=12) and running experimental (n=12), housed with free access to a wireless running wheel (Med Associates) for six weeks. For the sedentary group, the wheels were locked in the experiment. Therefore, this group of mice could not perform running exercises. Exercise activities of the running group were recorded for each animal for the investigation to ensure that each mouse was physically active. The recording was conducted using an automatic counter and Med Associates software. |
Sample Preparation:
Sampleprep ID: | SP003050 |
Sampleprep Summary: | Metabolites were extracted from feces, blood, brainstem and hippocampus. See Sample_preparation.pdf. |
Sampleprep Protocol Filename: | Sample_preparation.pdf |
Processing Storage Conditions: | Described in summary |
Extract Storage: | Described in summary |
Combined analysis:
Analysis ID | AN004809 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GCMS/MS-TQ8050 |
Column | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Triple quadrupole |
MS instrument name | Shimadzu GCMS/MS-TQ8050 |
Ion Mode | POSITIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH003634 |
Chromatography Summary: | 1 μL of each sample was injected in the GC-MS. Injection mode – split (15:1). The injection port temperature was set at 280°C, and helium was used as the carrier gas at a constant linear velocity of 39 cm/sec. Metabolites were separated using a GC temperature ramping program. The GC oven was programmed from 100°C to 280°C at a rate of 4°C/min. |
Methods Filename: | Chromatography.pdf |
Instrument Name: | Shimadzu GCMS/MS-TQ8050 |
Column Name: | Shimadzu SH-Rxi-5ms (30m x 0.25mm,0.25um) |
Column Temperature: | 280°C |
Flow Gradient: | NA |
Flow Rate: | 4°C/min |
Solvent A: | NA |
Solvent B: | NA |
Chromatography Type: | GC |
MS:
MS ID: | MS004555 |
Analysis ID: | AN004809 |
Instrument Name: | Shimadzu GCMS/MS-TQ8050 |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | MS acquisition: Metabolites were detected by setting the ion source filament energy to 70 eV, the ion source temperature - 200°C, and the scan range (mass-to-charge (m/z) 35 – 600 Da. Software/procedures used for feature assignments: Labsolution Shimadzu |
Ion Mode: | POSITIVE |