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MB Sample ID: SA328074
| Local Sample ID: | A30 |
| Subject ID: | SU003142 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU003142 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| A30 | SA328074 | FL038941 | AOM/DSS | Treatment |
Collection:
| Collection ID: | CO003135 |
| Collection Summary: | The colons were dissected and washed, and then flash-frozen in liquid N2 |
| Sample Type: | Colon |
Treatment:
| Treatment ID: | TR003151 |
| Treatment Summary: | The mice were intraperitoneally injected with 10 mg/kg azoxymethane (AOM) on the first day and then given 2% dextran sulfate sodium (DSS) for one week and water for two weeks (three cycles). And the stressed mice were confined in a ventilated 50 mL tube and stressed for 3-4 h daily. All mice were dissected 8 weeks after the start of the experiment. |
Sample Preparation:
| Sampleprep ID: | SP003148 |
| Sampleprep Summary: | Sample was thawed on ice. Take 50 mg of one sample and homogenize it with 1000 μl of ice-cold methanol/water (70%, v/v). Add cold steel balls to the mixture and homogenate for at 30 Hz for 3 min. Whirl the mixture for 1 min, and then centrifuge it with 12,000 rpm at 4°C for 10 min. The collected supernatant will be used for LC-MS/MS analysis. |
Combined analysis:
| Analysis ID | AN004964 | AN004965 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | SCIEX ExionLC AD | SCIEX ExionLC AD |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | count per second (cps) | count per second (cps) |
Chromatography:
| Chromatography ID: | CH003747 |
| Instrument Name: | SCIEX ExionLC AD |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40°C |
| Flow Gradient: | 0.4 mL/min |
| Flow Rate: | 95:5 V/V at 0 min, 5:95 V/V at 11.0 min, 5:95 V/V at 12.0 min, 95:5 V/V at 12.1 min, 95:5 V/V at 14.0 min |
| Solvent A: | water (0.04% acetic acid) |
| Solvent B: | acetonitrile (0.04% acetic acid) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS004704 |
| Analysis ID: | AN004964 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | In this study, we performed extensive targeted detection of metabolites in the colon using MRM scanning modalities. Therefore, we did not perform MS/MS data annotation. In the MRM mode, the quadrupole firstly screens the precursor ion (parent ion) of the target substance and excludes the ions corresponding to other molecular weight substances in order to preliminarily exclude the interference; the precursor ion is induced to ionize by the collision chamber and breaks off to form a lot of fragment ions, and the fragment ions are then filtered through the triple quadrupole to select a desired characteristic fragment ion and exclude the interference of non-target ions. We used MultiQuant software to analyze the data. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004705 |
| Analysis ID: | AN004965 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | In this study, we performed extensive targeted detection of metabolites in the colon using MRM scanning modalities. Therefore, we did not perform MS/MS data annotation. In the MRM mode, the quadrupole firstly screens the precursor ion (parent ion) of the target substance and excludes the ions corresponding to other molecular weight substances in order to preliminarily exclude the interference; the precursor ion is induced to ionize by the collision chamber and breaks off to form a lot of fragment ions, and the fragment ions are then filtered through the triple quadrupole to select a desired characteristic fragment ion and exclude the interference of non-target ions. We used MultiQuant software to analyze the data. |
| Ion Mode: | POSITIVE |
