Summary of Study ST000373

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000373
Study TitleMeasure change in metabolites based on diet and feeding status (part II)
Study TypeRegular
Study SummaryFlies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates
Institute
University of Michigan
DepartmentBiomedical Research Core
LaboratoryFacilities Metabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2016-04-20
Num Groups4
Total Subjects18
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HP4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000134
Project DOI:doi: 10.21228/M8HP4Z
Project Title:Fly Brain Studies
Project Summary:Measure change in metabolites according to diet and feeding state
Institute:University of Michigan
Department:MCDB LS&A
Laboratory:Dus Lab
Last Name:Dus
First Name:Monica
Address:Ann Arbor, MI
Email:mdus@umich.edu
Phone:347-891-2287

Subject:

Subject ID:SU000394
Subject Type:Animal
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Species Group:Insect

Factors:

Subject type: Animal; Subject species: Drosophila melanogaster (Factor headings shown in green)

mb_sample_id local_sample_id State
SA016968S00018443sated | Diet:high sugar
SA016969S00018447sated | Diet:high sugar
SA016970S00018446sated | Diet:high sugar
SA016971S00018444sated | Diet:high sugar
SA016972S00018445sated | Diet:high sugar
SA016973S00018451sated | Diet:normal
SA016974S00018449sated | Diet:normal
SA016975S00018450sated | Diet:normal
SA016976S00018448sated | Diet:normal
SA016977S00018458starved | Diet:high sugar
SA016978S00018459starved | Diet:high sugar
SA016979S00018460starved | Diet:high sugar
SA016980S00018457starved | Diet:high sugar
SA016981S00018452starved | Diet:normal
SA016982S00018453starved | Diet:normal
SA016983S00018454starved | Diet:normal
SA016984S00018455starved | Diet:normal
SA016985S00018456starved | Diet:normal
Showing results 1 to 18 of 18

Collection:

Collection ID:CO000388
Collection Summary:NONE
Sample Type:Tissues

Treatment:

Treatment ID:TR000408
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000401
Sampleprep Summary:none
Sampleprep Protocol Filename:Glycolysis-TCA-nucleotides-20150324.docx

Combined analysis:

Analysis ID AN000607
Analysis type MS
Chromatography type HILIC
Chromatography system Unspecified
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode NEGATIVE
Units pmol/µg tissue protein

Chromatography:

Chromatography ID:CH000433
Methods ID:DQM0108
Methods Filename:GlyTCA-ESN-HILIC-6490.m.zip
Instrument Name:Unspecified
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000540
Analysis ID:AN000607
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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