Summary of Study ST002023

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001285. The data can be accessed directly via it's Project DOI: 10.21228/M8MD8N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002023
Study Title	A targeted metabolomics study for assessing rodent thyroid toxicity
Study Summary	The thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.
Institute	Helmholtz Centre for Environmental Research
Department	Department of Molecular Systems biology
Laboratory	Functional Metabolomics
Last Name	Wang
First Name	Zhipeng
Address	Permoserstraße 15, Leipzig, Saxony, 04318, Germany
Email	zhipeng.wang@ufz.de
Phone	+49 341 235 1078
Submit Date	2021-12-20
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2022-12-20
Release Version	1

Select appropriate tab below to view additional metadata details:

Project:

Project ID:	PR001285
Project DOI:	doi: 10.21228/M8MD8N
Project Title:	A targeted metabolomics study for assessing rodent thyroid toxicity
Project Summary:	The thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.
Institute:	Helmholtz Centre for Environmental Research GmbH
Department:	Department of Molecular Systems biology
Laboratory:	Functional Metabolomics
Last Name:	Wang
First Name:	Zhipeng
Address:	Permoserstraße 15, Leipzig, Saxony, 04318, Germany
Email:	zhipeng.wang@ufz.de
Phone:	+49 341 235 1078

Subject:

Subject ID:	SU002105
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Short sample name
SA189446	L-A103-G11-M	L103 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189447	L-A104-G11-M	L104 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189448	L-A105-G11-M	L105 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189449	L-A108-G11-M	L108 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189450	L-A110-G11-M	L110 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189451	L-A112-G12-M	L112 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189452	L-A114-G12-M	L114 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189453	L-A116-G12-M	L116 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189454	L-A117-G12-M	L117 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189455	L-A118-G12-M	L118 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189456	L-A122-G13-M	L122 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189457	L-A123-G13-M	L123 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189458	L-A124-G13-M	L124 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189459	L-A127-G13-M	L127 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189460	L-A128-G13-M	L128 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189461	L-A12-G2-M	L12 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189462	L-A132-G14-M	L132 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189463	L-A135-G14-M	L135 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189464	L-A136-G14-M	L136 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189465	L-A139-G14-M	L139 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189466	L-A140-G14-M	L140 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189467	L-A142-G15-M	L142 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189468	L-A143-G15-M	L143 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189469	L-A144-G15-M	L144 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189470	L-A145-G15-M	L145 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189471	L-A148-G15-M	L148 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189472	L-A15-G2-M	L15 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189473	L-A16-G2-M	L16 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189474	L-A17-G2-M	L17 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189475	L-A18-G2-M	L18 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189476	L-A22-G3-M	L22 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189477	L-A23-G3-M	L23 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189478	L-A25-G3-M	L25 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189479	L-A28-G3-M	L28 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189480	L-A2-G1-M	L2 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:2 weeks \| Recovery:0 weeks
SA189481	L-A30-G3-M	L30 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189482	L-A32-G4-M	L32 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189483	L-A33-G4-M	L33 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189484	L-A34-G4-M	L34 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189485	L-A36-G4-M	L36 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189486	L-A38-G4-M	L38 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189487	L-A43-G5-M	L43 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189488	L-A45-G5-M	L45 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189489	L-A47-G5-M	L47 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189490	L-A48-G5-M	L48 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189491	L-A49-G5-M	L49 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189492	L-A4-G1-M	L4 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:2 weeks \| Recovery:0 weeks
SA189493	L-A52-G6-M	L52 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:0 weeks
SA189494	L-A53-G6-M	L53 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:0 weeks
SA189495	L-A55-G6-M	L55 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:0 weeks
SA189496	L-A56-G6-M	L56 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:0 weeks
SA189497	L-A58-G6-M	L58 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:0 weeks
SA189498	L-A5-G1-M	L5 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:2 weeks \| Recovery:0 weeks
SA189499	L-A62-G7-M	L62 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189500	L-A65-G7-M	L65 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189501	L-A66-G7-M	L66 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189502	L-A67-G7-M	L67 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189503	L-A6-G1-M	L6 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:2 weeks \| Recovery:0 weeks
SA189504	L-A70-G7-M	L70 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189505	L-A72-G8-M	L72 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189506	L-A73-G8-M	L73 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189507	L-A74-G8-M	L74 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189508	L-A76-G8-M	L76 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189509	L-A80-G8-M	L80 \| Organ:Liver \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189510	L-A82-G9-M	L82 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189511	L-A85-G9-M	L85 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189512	L-A88-G9-M	L88 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189513	L-A89-G9-M	L89 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189514	L-A8-G1-M	L8 \| Organ:Liver \| Treatment:Control \| Concentration:- \| Period:2 weeks \| Recovery:0 weeks
SA189515	L-A90-G9-M	L90 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189516	L-A92-G10-M	L92 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189517	L-A93-G10-M	L93 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189518	L-A94-G10-M	L94 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189519	L-A96-G10-M	L96 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189520	L-A98-G10-M	L98 \| Organ:Liver \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:0 weeks
SA189521	S-A103-G11-M	S103 \| Organ:Thyroid \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189522	S-A104-G11-M	S104 \| Organ:Thyroid \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189523	S-A105-G11-M	S105 \| Organ:Thyroid \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189524	S-A108-G11-M	S108 \| Organ:Thyroid \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189525	S-A110-G11-M	S110 \| Organ:Thyroid \| Treatment:Control \| Concentration:- \| Period:4 weeks \| Recovery:2 weeks
SA189526	S-A112-G12-M	S112 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189527	S-A114-G12-M	S114 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189528	S-A116-G12-M	S116 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189529	S-A117-G12-M	S117 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189530	S-A118-G12-M	S118 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189531	S-A122-G13-M	S122 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189532	S-A123-G13-M	S123 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189533	S-A124-G13-M	S124 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189534	S-A127-G13-M	S127 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189535	S-A128-G13-M	S128 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:2400 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189536	S-A12-G2-M	S12 \| Organ:Thyroid \| Treatment:Phenytoin \| Concentration:300 ppm \| Period:2 weeks \| Recovery:0 weeks
SA189537	S-A132-G14-M	S132 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189538	S-A135-G14-M	S135 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189539	S-A136-G14-M	S136 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189540	S-A139-G14-M	S139 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189541	S-A140-G14-M	S140 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:5 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189542	S-A142-G15-M	S142 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189543	S-A143-G15-M	S143 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189544	S-A144-G15-M	S144 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks
SA189545	S-A145-G15-M	S145 \| Organ:Thyroid \| Treatment:6-propyl-2-thiouracil \| Concentration:50 ppm \| Period:4 weeks \| Recovery:2 weeks

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Collection:

Collection ID:	CO002098
Collection Summary:	The animal experiment was done by our cooperative partner BASF. The liver and thyroid tissue samples were delivered to us with the protection of dry ice. And all samples was stored immediately under -80℃ until further analysis.
Sample Type:	Tissues

Treatment:

Treatment ID:	TR002117
Treatment Summary:	Two well-described test chemicals, i.e. Phenytoin and Propylthiouracil, were administered via the diet at each two concentrations to male Wistar rats for 2 and 4 weeks. In addition, a recovery period of 2 weeks without test substance application was included in order to monitor reversibility of the induced effects. Phenytoin was administered concentrations of 300 and 2400 ppm, Propylthiouracil was administered at concentrations of 5 and 50 ppm. Control animals received maintenance diet only without inclusion of any test substance.

Treatment ID:

TR002117

Treatment Summary:

Two well-described test chemicals, i.e. Phenytoin and Propylthiouracil, were administered via the diet at each two concentrations to male Wistar rats for 2 and 4 weeks. In addition, a recovery period of 2 weeks without test substance application was included in order to monitor reversibility of the induced effects. Phenytoin was administered concentrations of 300 and 2400 ppm, Propylthiouracil was administered at concentrations of 5 and 50 ppm. Control animals received maintenance diet only without inclusion of any test substance.

Sample Preparation:

Sampleprep ID:	SP002111
Sampleprep Summary:	Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.

Sampleprep ID:

SP002111

Sampleprep Summary:

Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.

Combined analysis:

Analysis ID	AN003293
Analysis type	MS
Chromatography type	Unspecified
Chromatography system	Agilent 1290 Infinity
Column	MxP Quant 500 System
MS Type	ESI
MS instrument type	QTRAP
MS instrument name	ABI Sciex 5500 QTrap
Ion Mode	UNSPECIFIED
Units	µM

Chromatography:

Chromatography ID:	CH002432
Instrument Name:	Agilent 1290 Infinity
Column Name:	MxP Quant 500 System
Chromatography Type:	Unspecified

MS:

MS ID:	MS003063
Analysis ID:	AN003293
Instrument Name:	ABI Sciex 5500 QTrap
Instrument Type:	QTRAP
MS Type:	ESI
MS Comments:	Both FIA and LC methods were used. Both positive and negative were used. The MS acquisition and Data processing were done following the manufacturer's instructions. from Biocrates
Ion Mode:	UNSPECIFIED