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MB Sample ID: SA011150

Local Sample ID:CKT1
Subject ID:SU000261
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Biosource Or Supplier:ATCC
Cell Strain Details:HepG2
Subject Comments:NA
Cell Primary Immortalized:Immortalized
Cell Passage Number:NA
Cell Counts:NA
Species Group:Human

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Combined analysis:

Analysis ID AN000373 AN000374
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1200 Agilent 1200
Column ACE 5 C8-300 (100 x 2.1mm) ACE 5 C8-300 (100 x 2.1mm)
MS Type ESI ESI
MS instrument type FT-ICR-MS FT-ICR-MS
MS instrument name Bruker SolariX FT-ICR-MS Bruker SolariX FT-ICR-MS
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000270
Methods Filename:jac8lipidneg.m
Chromatography Comments:jalcms1lipneg.m
Instrument Name:Agilent 1200
Column Name:ACE 5 C8-300 (100 x 2.1mm)
Flow Rate:0.1 mL/min
Internal Standard:25 g C15:0 PE, C17:0 PC and C71:1 TAG
Solvent A:100% water; 0.1% acetic acid; 2 mM ammonium acetate
Solvent B:50% acetonitrile/50% isopropanol; 0.1% formic acid; 10 mM ammonium acetate
Analytical Time:60 min
Chromatography Type:Reversed phase
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