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MB Sample ID: SA012095
| Local Sample ID: | QE1_REP_18_10 |
| Subject ID: | SU000289 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | 8 or 24 week |
| Gender: | male |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000431 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | nmol/g liver protein |
Chromatography:
| Chromatography ID: | CH000301 |
| Chromatography Summary: | Waters Acquity BEH C18 (2.1 x 50 mm, 1.7 um) with guard was used to separate compounds with mobile phases made of isopropanol, acetonitrile, and water. |
| Methods Filename: | REP_18 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 30 C |
| Flow Gradient: | 32% B to 98% B over 18 mins |
| Flow Rate: | 0.5 mL/min |
| Injection Temperature: | 4 C |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Analytical Time: | 18 mins |
| Capillary Voltage: | 350 C |
| Time Program: | 0-1 min: 32%B --> 1-1.5 min: 40% B --> 4 min: 45% B --> 5 min: 50% B --> 8 min: 60% B --> 11 min: 70% B --> 14 min: 80% B --> 16-17 min: 100%B --> 18 min: 32%B --> 21 min: 32% B |
| Strong Wash Solvent Name: | 1:1:1 isopropanol:acetonitrile:water |
| Strong Wash Volume: | 100 uL |
| Chromatography Type: | Reversed phase |
