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MB Sample ID: SA154477

Local Sample ID:CTRL_1
Subject ID:SU001750
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN002731
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Accela 1250
Column Thermo Accucore C18 (100 x 2.1mm,2.6um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units pmoles

Chromatography:

Chromatography ID:CH002017
Chromatography Summary:Samples were analyzed with a Quantum Ultra triple quadrupole mass spectrometer connected to an Accela HPLC and Accela autosampler using a solvent gradient. Ceramides identity was achieved through MRM analysis with soft fragmentation. Quantitative analysis is based on calibration curves generated for each ceramide. J. Bielawski et al. / Methods 39 (2006) 82–91
Instrument Name:Thermo Accela 1250
Column Name:Thermo Accucore C18 (100 x 2.1mm,2.6um)
Chromatography Type:Reversed phase
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