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MB Sample ID: SA327743
Local Sample ID: | BR_Control_000_1 |
Subject ID: | SU003138 |
Subject Type: | Bacteria |
Subject Species: | Pseudomonas aeruginosa |
Taxonomy ID: | 287 |
Genotype Strain: | CW41 |
Age Or Age Range: | NA |
Weight Or Weight Range: | NA |
Height Or Height Range: | NA |
Gender: | Not applicable |
Cell Strain Details: | hypermutable clinical isolate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004958 | AN004959 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH003742 |
Chromatography Summary: | ZIC-pHILIC chromatography at ph9, biological replicates 3-5 |
Methods Filename: | Metabolomics_pHILIC_Parkville_v1.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
Column Pressure: | 60 bar at starting conditions. 180 bar at %A |
Column Temperature: | 25 C |
Flow Gradient: | 0 min - 80%B, 15 min - 50%B, 18 min - 5%B, 21 min - 5%B, 24 min - 80%B, 32 min - 80%B |
Flow Rate: | 0.3 ml/min |
Injection Temperature: | 4 C |
Sample Injection: | 10 uL |
Solvent A: | 20 mM ammonium carbonate |
Solvent B: | acetonitrile |
Analytical Time: | 32 min |
Capillary Voltage: | 4 kV |
Washing Buffer: | syringe wash 50% IPA |
Sample Loop Size: | 25 uL |
Sample Syringe Size: | 25 uL |
Randomization Order: | yes |
Chromatography Type: | HILIC |