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MB Sample ID: SA327833
| Local Sample ID: | M_Control_023_3 |
| Subject ID: | SU003138 |
| Subject Type: | Bacteria |
| Subject Species: | Pseudomonas aeruginosa |
| Taxonomy ID: | 287 |
| Genotype Strain: | CW41 |
| Age Or Age Range: | NA |
| Weight Or Weight Range: | NA |
| Height Or Height Range: | NA |
| Gender: | Not applicable |
| Cell Strain Details: | hypermutable clinical isolate |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004958 | AN004959 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak height | peak height |
Chromatography:
| Chromatography ID: | CH003742 |
| Chromatography Summary: | ZIC-pHILIC chromatography at ph9, biological replicates 3-5 |
| Methods Filename: | Metabolomics_pHILIC_Parkville_v1.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Merck SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
| Column Pressure: | 60 bar at starting conditions. 180 bar at %A |
| Column Temperature: | 25 C |
| Flow Gradient: | 0 min - 80%B, 15 min - 50%B, 18 min - 5%B, 21 min - 5%B, 24 min - 80%B, 32 min - 80%B |
| Flow Rate: | 0.3 ml/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 10 uL |
| Solvent A: | 20 mM ammonium carbonate |
| Solvent B: | acetonitrile |
| Analytical Time: | 32 min |
| Capillary Voltage: | 4 kV |
| Washing Buffer: | syringe wash 50% IPA |
| Sample Loop Size: | 25 uL |
| Sample Syringe Size: | 25 uL |
| Randomization Order: | yes |
| Chromatography Type: | HILIC |
