Summary of Study ST002924
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001816. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX5S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002924 |
Study Title | Gut sphingolipid metabolites in infants with atopic dermatitis associated with food allergy - Part 2 |
Study Summary | This study determines sphingolipids and diacylglycerols from infant feces to explore the lipid changes with food allergy in atopic dermatitis. Food allergy (FA) may be present in the range of 20–80% in atopic dermatitis (AD). Food sensitization through the skin can cause FA due to damage to the skin barrier, and failure to acquire tolerance to food allergens in the gut can equally cause the development of FA. Gut metabolites can influence the physical gut barrier and intestinal homeostasis. Therefore, it is possible that gut metabolites related to gut immunity play an important role in the development of FA. Sphingolipids are key factors in cell inflammatory response and affect gut epithelial cells and skin barrier integrity and function. FA is associated with a marked decrease in serum sphingolipid levels. However, there are no reports of FA-associated gut sphingolipid metabolites in infants by targeted metabolomics. In our previous study, we showed that when FA is present in various phenotypes of AD in early life, it might be associated with the later development of asthma. The discovery of a biomarker that can distinguish the phenotypes that accompany AD and FA from other AD phenotypes is therefore expedient. Consequently, we aimed to investigate whether FA in AD infants. can be classified as gut sphingolipid metabolites using targeted metabolomics. |
Institute | Asan Medical Center |
Last Name | Yoo |
First Name | Hyun Ju |
Address | 88, Olympic-ro, 43-gil, Songpa-gu, Seoul, Seoul, 05505, Korea, South |
yoohyunju@amc.seoul.kr | |
Phone | 02-3010-4029 |
Submit Date | 2023-10-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | rdb |
Analysis Type Detail | LC-MS |
Release Date | 2023-11-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004794 | AN004795 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) |
MS Type | ESI | ESI |
MS instrument type | QTRAP | QTRAP |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | POSITIVE |
Units | pmol/mg | pmol/mg |
Chromatography:
Chromatography ID: | CH003625 |
Chromatography Summary: | Ceramides, sphingomyelines, sphinganine, sphingosine |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) |
Column Temperature: | 35 |
Flow Gradient: | 50 % of B at 0 min, 50 % of B for 5 min, 50-70 % of B for 3 min, 70 % of B for 7 min, 70-90 % of B for 7 min, 90 % of B for 3 min, 90-50 % of B for 0.1 min, 50 % of B for 4.9 min |
Flow Rate: | 200 uL/min |
Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran (500/200/300, v/v/v) |
Solvent B: | 5 mM ammonium formate/MeOH/ tetrahydrofuran (100/200/700, v/v/v) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003626 |
Chromatography Summary: | diacylglycerols |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Agilent Pursuit 5 C18 (150 x 2.0 mm, 5um) |
Column Temperature: | 35 |
Flow Gradient: | 90 % of B for 0 min, 90 % of B for 6 min, 90 to 95 % of B for 0.6 min, 95 % of B for 3.4 min, 95 to 90 % of B for 0.1 min, and 90 % of B for 1.9 min |
Flow Rate: | 200 uL/min |
Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran (500/200/300, v/v/v) |
Solvent B: | 5 mM ammonium formate/MeOH/ tetrahydrofuran (100/200/700, v/v/v) |
Chromatography Type: | Reversed phase |