Summary of Study ST000592
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000384. The data can be accessed directly via it's Project DOI: 10.21228/M8DP41 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000592 |
Study Title | Uninfected Macaca mulatta exposed to pyrimethamine to produce and integrate clinical, hematological, and omics control measures. |
Study Summary | Uninfected, malaria-naive, male rhesus macaques (Macaca mulatta), approximately two years of age, were inoculated intravenously with a preparation of salivary gland material derived from non-infected Anopheles dirus and profiled for clinical, hematological, functional genomic, lipidomic, proteomic, and metabolomic measurements. Samples were generated and analyzed to investigate the effects of the pharmacological intervention with the anti-malarial drug pyrimethamine on normal individuals. The experiment was designed for 100 days plus a follow-up period, with pyrimethamine administered at three different time points to coincide with the predicted treatment days of experimentally infected rhesus macaques. Capillary blood samples were collected daily for the measurement of CBCs and reticulocytes. Capillary blood samples were collected every other day to obtain plasma for metabolomic analysis. Venous blood samples and bone marrow aspirates were collected at seven time points before and after three rounds of drug administration for functional genomic, proteomic, and lipidomic analyses. Within the MaHPIC, this project is known as 'Experiment 13'. This dataset was produced by Dean Jones at Emory University. The following contributed to the creation of this dataset: The MaHPIC Consortium, John Barnwell, Monica Cabrera, Jeremy D. DeBarry, Mary Galinski, Trenton Hoffman, Jay Humphrey, Jianlin Jiang, Chet Joyner, Nicolas Lackman, Stacey Lapp, Esmeralda Meyer, Alberto Moreno, Mustafa Nural, and Suman Pakala. The experimental design and protocols for this study were approved by the Emory University Institutional Animal Care and Use Committee (IACUC). |
Institute | Emory University |
Department | School of Medicine, Vaccine Center at Yerkes |
Last Name | Galinksi |
First Name | Mary |
Address | Emory University, Yerkes National Primate Research Center, 954 Gatewood Rd, Room 003, Atlanta, GA 30329 |
mahpic@emory.edu | |
Phone | none |
Submit Date | 2017-04-05 |
Total Subjects | 6 |
Study Comments | 31 samples, "The experimental design and protocols for this study were approved by the Emory University Institutional Animal Care and Use Committee (IACUC). These results are a product of a consortium of researchers known as the Malaria Host Pathogen Interaction Center (MaHPIC). For more information on the MaHPIC, please visit http://www.systemsbiology.emory.edu/ . Within the MaHPIC, these data were collected as part of 'Experiment 13' (E13). To access other publicly available results from E13 and other MaHPIC Experiments, including clinical results (specifics on drugs administered, diet, and veterinary interventions), and other omics, visit http://plasmodb.org/plasmo/mahpic.jsp . This page will be updated as datasets are released to the public. " Publication: (PMC4233942) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4233942/ |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-07-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000907 | AN000908 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Ion exchange |
Chromatography system | Thermo Accela CTC | Thermo Accela CTC |
Column | Higgins C18 (100 x 2.1mm,5um) | Hamilton Anion Exchange (100 x 2.1mm,7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | POSITIVE |
Units | unspecified | unspecified |
Chromatography:
Chromatography ID: | CH000644 |
Chromatography Summary: | untargeted chromatography (C18 column) |
Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
Instrument Name: | Thermo Accela CTC |
Column Name: | Higgins C18 (100 x 2.1mm,5um) |
Column Pressure: | 200 Bar (max) |
Column Temperature: | 30 |
Flow Gradient: | A%: 0-10mins = 5%; B%: 0-2mins = 35%, 2-7mins = gradient to 95%, 7-10mins = 95%; C%: 0-2mins = 60%, 2-7mins = gradient to 0%, 7-10mins = 0% |
Flow Rate: | 350 μL/min |
Injection Temperature: | 4C |
Internal Standard: | Caffeine, Diethyl-toluamide |
Internal Standard Mt: | 1.5 min; 6-6.6 min |
Sample Injection: | 10μL |
Sampling Cone: | ESI: HESI probe with S-lens combination |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Analytical Time: | 10 minutes |
Capillary Voltage: | 4.6 kV |
Oven Temperature: | 45C |
Running Voltage: | 4.6 kV |
Weak Wash Solvent Name: | H20 with 10% methanol |
Sample Syringe Size: | 20μL |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000645 |
Chromatography Summary: | anion exchange chromatography |
Methods Filename: | Metab_Orbitrap_Setup_v1_May_17_2013.docx |
Instrument Name: | Thermo Accela CTC |
Column Name: | Hamilton Anion Exchange (100 x 2.1mm,7um) |
Column Pressure: | 200 Bar (max) |
Column Temperature: | 30 |
Flow Gradient: | A%: 0-2mins = 5%, 2-7mins = gradient to 50%, 7-10mins = 50%; B%: 0-10mins = 50%; C%: 0-2mins = 45%, 2-7mins = gradient to 0%, 7-10mins = 0% |
Flow Rate: | 350 μL/min |
Injection Temperature: | 4C |
Internal Standard: | Cystine; cysteine-glutathione disulfide; carboxymethyl-cysteine (very low); glutathione disulfide (+1 and +2); carboxymethyl-glutathione |
Internal Standard Mt: | 1-1.5 min; 2-4 min; 3.5-5 min; 7-8 min; 7-8 min |
Sample Injection: | 10μL |
Sampling Cone: | ESI: HESI probe with S-lens combination |
Solvent A: | 100% water; 0.2% formic acid |
Solvent B: | 100% acetonitrile |
Analytical Time: | 10 minutes |
Capillary Voltage: | 4.6 kV |
Oven Temperature: | 45C |
Running Voltage: | 4.6 kV |
Weak Wash Solvent Name: | H20 with 10% methanol |
Sample Syringe Size: | 20μL |
Chromatography Type: | Ion exchange |