Summary of Study ST000466
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000358. The data can be accessed directly via it's Project DOI: 10.21228/M8S302 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000466 |
Study Title | Streptococcus mutans organic acids/amino acid profiles, effect of oxidative stress; pilot |
Study Type | Amino acid/organic acid profiles of wild-type and mutant strain in response to H2O2 treatment over time. |
Study Summary | S. mutans UA159 and ΔspxA1 will be grown in BHI to OD600 = 0.4. Each culture will be split in to 4 samples. For the first sample, 3E10 cells will immediately be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. The remaining three samples will be treated with H2O2 to 0.5 mM, and the harvesting process repeated after 15, 30 and 60 minutes. |
Institute | University of Florida |
Department | SECIM |
Laboratory | Lemos-Abranches |
Last Name | Hardin |
First Name | Emily |
Address | 1395 Center Drive, PO Box 100424, Gainesville, FL 32610 |
ehardin@ufl.edu | |
Phone | 352-273-8844 |
Submit Date | 2016-09-08 |
Num Groups | 6 |
Total Subjects | 6 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
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Collection:
Collection ID: | CO000481 |
Collection Summary: | Upon reaching OD600 = 0.4, the culture is split into 4 samples. Three are treated with H2O2 (0.5 mM). The other is harvested by filtration onto 0.45uM nitrocellulose membrane. The cells will be scraped off of the membrane into ice-cold PBS (~1 mL), centrifuged for 30 seconds at 12K RPM, liquid aspirated and stored at -80C until sending for analysis. This process will be repeated with the remaining three samples after 15, 30 and 60 minutes treatment with H2O2. |
Sample Type: | Bacterial cells |