Summary of Study ST001609
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001034. The data can be accessed directly via it's Project DOI: 10.21228/M81X28 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001609 |
Study Title | Comparative metabolomics analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p (part-I) |
Study Summary | To gather more in-depth knowledge of the Mtl1p mechanosensor's role in Saccharomyces cerevisiae metabolism, we conducted a comparative metabolomic analysis of two Saccharomyces cerevisiae strains: the wild type and mtl1Δ, which carries a deletion of the mechanosensor Mtl1p. Both strains were grown under normal conditions at 27°C. The most significant metabolic changes between these strains were related to amino acid metabolism, purine metabolism, and carboxylic acid metabolism. |
Institute | University of Puerto Rico, Medical Sciences Campus |
Last Name | Chorna |
First Name | Nataliya |
Address | University of Puerto Rico, Medical Sciences Campus, Department of Biochemistry, Main Building, 6th Floor, Room A-632, San Juan, PR 00935 |
nataliya.chorna@upr.edu | |
Phone | 787-758-2525 ext 1640 |
Submit Date | 2020-11-27 |
Num Groups | 2 |
Total Subjects | 26 |
Raw Data Available | Yes |
Raw Data File Type(s) | qgd |
Analysis Type Detail | GC-MS |
Release Date | 2020-12-10 |
Release Version | 1 |
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Collection:
Collection ID: | CO001679 |
Collection Summary: | Cells were grown at 27°C, 210 rpm in 5 mL of complete synthetic medium (CSM, 0.67% Nitrogen base without amino acids and ammonium sulfate, 2% glucose). The next day, cells were replaced with fresh CSM and grow at 27°C to reach an OD of 0.6 - 0.7. 25 ml of each sample were harvested by centrifugation at 3,838 x g for 5 min at 4°C, washed with 1 ml ice cold sterile water, quenched using ice cold methanol, and stored at -80°C. |
Sample Type: | Yeast cells |