Summary of Study ST003170

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001970. The data can be accessed directly via it's Project DOI: 10.21228/M8314P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003170
Study TitleMetabolomics of Murine WT or MCJ KO CD19-BBz CD8 CAR-T cells rest in medium
Study SummaryMCJ/DnaJC15 is an endogenous negative regulator of Complex I and mitochondrial respiration. The goal of these experiments are to characterize the metabolic profile of murine WT or MCJ KO CD8 CD19-BBz CAR-T cells in cytokine-deprived condition. The CAR-T cells were expanded with IL-2 (60IU/ml) for 3 expansions (6 days) and rested in the medium for 48 hours.
Institute
University of Colorado School of Medicine
LaboratoryLaboratory of Angelo D'Alessandro in collaboration with Mercedes Rincon
Last NameCendali
First NameFrancesca
Address13199 East Montview Boulevard, Aurora, CO, 80045, USA
Emailfrancesca.cendali@cuanschutz.edu
Phone3037246131
Submit Date2024-04-11
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-05-02
Release Version1
Francesca Cendali Francesca Cendali
https://dx.doi.org/10.21228/M8314P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO003282
Collection Summary:Murine CD8 cells were isolated from lymph nodes and spleen using negative selection with CD8+ T cell isolation kit (for generation of CAR-T cells) (Miltenyi, order# 130-104-075) or positive selection (for OT-I CD8 cells) with CD8 (Ly-2) Microbeads (Miltenyi, order# 130-117-044). Mouse CD8 cells were activated with plate-bound anti-CD3 (5 g/ml) and soluble anti-CD28 mAbs (1 g/ml) for 48h for WT and cMCJ KO CD8 studies and OT-I CD8 cells studies.
Sample Type:T-cells
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