Summary of Study ST000599
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000384. The data can be accessed directly via it's Project DOI: 10.21228/M8DP41 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000599 |
Study Title | Metabolomics measures of Macaca mulatta infected with Plasmodium coatneyi Hackeri strain |
Study Type | Longitudinal parasite infection and treatment of multiple individuals |
Study Summary | ATTENTION: The dataset package associated with this study is out of date. Please refer to https://www.ebi.ac.uk/metabolights/MTBLS518 for the latest and final version of all files. Malaria-naive male rhesus macaques (Macaca mulatta), approximately four years of age, were inoculated intravenously with salivary gland sporozoites produced and isolated at the Centers for Disease Control and Prevention from multiple Anopheles species (An. dirus, An. gambiae, and An. stephensi) and then profiled for clinical, hematological, parasitological, immunological, functional genomic, lipidomic, proteomic, and metabolomic measurements. The experiment was designed for 100 days, and pre- and post-100 day periods to prepare subjects and administer curative treatments respectively. The anti-malarial drug artemether was subcuratively administered to all subjects at the initial peak of infection, one out of the five macaques received four additional subcurative treatments for subsequent recrudescence peaks. The experimental infection in one subject was ineffective but the macaque was followed-up for the same period of 100 days. The different clinical phases of the infection were clinically determined for each subject. Blood-stage curative doses of artemether were administered to all subjects at the end of the study. Capillary blood samples were collected daily for the measurement of CBCs, reticulocytes, and parasitemias. Capillary blood samples were collected every other day to obtain plasma for metabolomic analysis. Venous blood and bone marrow samples were collected at seven time points for functional genomic, proteomic, lipidomic, and immunological analyses. Within the MaHPIC, this project is known as ‘Experiment 03’. This dataset was produced by Dean Jones at Emory University. The experimental design and protocols for this study were approved by the Emory University Institutional Animal Care and Use Committee (IACUC). |
Institute | Emory University |
Department | School of Medicine, Vaccine Center at Yerkes |
Last Name | Galinski |
First Name | Mary |
Address | Emory University, Yerkes National Primate Research Center, 954 Gatewood Rd, Room 003, Atlanta, GA 30329 |
mahpic@emory.edu | |
Phone | N/A |
Submit Date | 2017-03-23 |
Num Groups | 6 |
Total Subjects | 331 |
Study Comments | The experimental design and protocols for this study were approved by the Emory University Institutional Animal Care and Use Committee (IACUC). These results are a product of a consortium of researchers known as the Malaria Host Pathogen Interaction Center (MaHPIC). Contributors include: Monica Cabrera, Jeremy D. DeBarry, Mary G. Galinski, Jay Humphrey, Dean Jones, Ebru Karpuzoglu, Jessica C. Kissinger, Regina Joice, Esmeralda Meyer, Vishal Nayak, Mustafa Nural, Suman Pakala, ViLinh Tran, Karan Uppal, Loukia Williams. For more information on the MaHPIC, please visit http://www.systemsbiology.emory.edu/ . Within the MaHPIC, these data were collected as part of 'Experiment 03' (E03). To access other publicly available results from E03 and other MaHPIC Experiments, including clinical results (specifics on drugs administered, diet, and veterinary interventions), and other omics, visit http://plasmodb.org/plasmo/mahpic.jsp . This page will be updated as datasets are released to the public. |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2017-07-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
Collection ID: | CO000616 |
Collection Summary: | Every Other Day Collection | Time Point 1 Baseline: Uninfected control for each Non-Human Primate (NHP). | Time Point 2 Peak of infection: Peak of infection determined by clinical and parasitological assessment for each NHP. The time point is intended to characterize the host immune response and capture multi-omics data during the peak of parasitemias when the NHP is experiencing clinical signs of disease that are commonly severe in P. coatneyi infections. NHPs were monitored based on clinical and hematological parameters, and clinical interventions with artemether were performed as necessary. | Time Point 3 Post-peak: Observations 7 days after peak infection and sub-curative treatment. | Time Point 4 Recrudescence Peak: This time point tested expectations of a drop in hemoglobin levels and increase in parasitemias due to recrudescence from a chronic infection. After sample collection, NHPs received sub-curative treatment with artemether based on clinical and hematological parameters. | Time Point 5 Post-peak: Chronic infection with low levels of persistent parasitemia that may be below detectable levels. | Time Point 6 Recrudescence Peak: Same goal as time point 4 for 2nd second recrudescence peak. | Time Point 7 Post-peak: Observations of NHP response after a second recrudescence in a chronic phase with low levels of persistent parasitemia. |
Sample Type: | Whole blood|Whole blood|Whole blood|Whole blood|Whole blood|Whole blood|Whole blood|Whole blood |
Collection Method: | Ear Prick|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous|Intravenous |
Collection Frequency: | Every Other Day|Once|Once|Once|Once|Once|Once|Once |
Blood Serum Or Plasma: | Plasma|Plasma|Plasma|Plasma|Plasma|Plasma|Plasma|Plasma |