Summary of Study ST002060
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001304. The data can be accessed directly via it's Project DOI: 10.21228/M8570V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002060 |
Study Title | Pollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage |
Study Type | Untargeted Metabolomics |
Study Summary | In this study, we investigated the differential metabolic pathway enrichment among mature, hydrated, and germinated pollen using untargeted metabolomics analysis. Integration of publicly available transcriptome with presented metabolome data revealed starch and sucrose metabolism was significantly increased during pollen hydration and germination. The alterations in central metabolism focusing on sugar, fatty acids, and lipids were analyzed in detail. Several metabolites, including palmitic acid, oleic acid, linolenic acid, quercetin, luteolin/kaempferol, and γ-aminobutyric acid (GABA), were elevated in the hydrated pollen, suggesting a potential role in activating pollen tube emergence. The metabolite levels of mature, hydrated, and germinated pollen, presented in this work provide insights on the molecular basis of pollen germination. |
Institute | University of Illinois Urbana-Champaign |
Department | Department of Plant Biology |
Laboratory | Li-Qing Chen Lab |
Last Name | Kambhampati |
First Name | Shrikaar |
Address | 975 North Warson Road, St. Louis, MO 63132 |
shrikaar.k@gmail.com | |
Phone | 3144025550 |
Submit Date | 2022-01-17 |
Num Groups | 3 |
Total Subjects | 12 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2022-02-07 |
Release Version | 1 |
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Collection:
Collection ID: | CO002135 |
Collection Summary: | The Arabidopsis Col-0 plants were grown under controlled temperature (22°C) with a 16-h light (100-150 µmol m-2 s-1)/ 8-h dark photoperiod. Mature pollen grains from the fully opened flowers were collected from more than 1000 plants using a vacuum cleaner method (Johnson-Brousseau and McCormick, 2004) at around 5 hours into the light period. For mature pollen samples, collected pollen grains were resuspended in 2 ml of ice-cold Pollen Isolation Buffer (PIB, composed of 100 mM NaPO4, pH 7.5, 1 mM EDTA, and 0.1% (v/v) Triton X-100) right after collection followed by centrifuging at 15,000 g for 1 min (4°C). For hydrated pollen and germinated pollen samples, mature pollen grains were germinated in vitro according to a previously described pollen transcriptome study (Wang et al., 2008). In brief, pollen pellets were washed with 1 ml of liquid Pollen Germination Medium (PGM, composed of 15% (w/v) sucrose, 1.5 mM boric acid, 0.8 mM MgSO4, 1 mM KCl, 5 mM MES, 0.05% (w/v) lactalbumin hydrolysate, 10 µM myo-inositol, 5 mM CaCl2) before they were resuspended in 30 µl of liquid PGM and subsequently cultured in Petri dishes (35 mm in diameter). A 70 µm mesh was used to cover the pollen droplet to create a thin layer for optimal germination for each Petri dish. The Petri dishes were covered and placed in the dark for 45 min or 4 h and collected as hydrated pollen or germinated pollen, respectively. All pollen samples were washed by 1 ml ice-cold ddH2O three times before being stored in a -80 °C freezer. |
Collection Protocol Filename: | PlantGrowth_PollenCollection.docx |
Sample Type: | Plant |
Collection Method: | Flash frozen in Liquid N2 |
Collection Location: | University of Illinois, Urbana-Champain |
Storage Conditions: | -80℃ |