Summary of Study ST002060

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001304. The data can be accessed directly via it's Project DOI: 10.21228/M8570V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002060
Study TitlePollen metabolomics using Arabidopsis thaliana: Comparison of pollen at mature, hydration and germination stage
Study TypeUntargeted Metabolomics
Study SummaryIn this study, we investigated the differential metabolic pathway enrichment among mature, hydrated, and germinated pollen using untargeted metabolomics analysis. Integration of publicly available transcriptome with presented metabolome data revealed starch and sucrose metabolism was significantly increased during pollen hydration and germination. The alterations in central metabolism focusing on sugar, fatty acids, and lipids were analyzed in detail. Several metabolites, including palmitic acid, oleic acid, linolenic acid, quercetin, luteolin/kaempferol, and γ-aminobutyric acid (GABA), were elevated in the hydrated pollen, suggesting a potential role in activating pollen tube emergence. The metabolite levels of mature, hydrated, and germinated pollen, presented in this work provide insights on the molecular basis of pollen germination.
Institute
University of Illinois Urbana-Champaign
DepartmentDepartment of Plant Biology
LaboratoryLi-Qing Chen Lab
Last NameKambhampati
First NameShrikaar
Address975 North Warson Road, St. Louis, MO 63132
Emailshrikaar.k@gmail.com
Phone3144025550
Submit Date2022-01-17
Num Groups3
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-02-07
Release Version1
Shrikaar Kambhampati Shrikaar Kambhampati
https://dx.doi.org/10.21228/M8570V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Arabidopsis thaliana (Factor headings shown in green)

mb_sample_id local_sample_id Stage Chromatography Polarity
SA193945Germinated_HILIC_Neg_3Germination HILIC Negative
SA193946Germinated_HILIC_Neg_1Germination HILIC Negative
SA193947Germinated_HILIC_Neg_4Germination HILIC Negative
SA193948Germinated_HILIC_Neg_2Germination HILIC Negative
SA193949Germinated_HILIC_Pos_4Germination HILIC Positive
SA193950Germinated_HILIC_Pos_1Germination HILIC Positive
SA193951Germinated_HILIC_Pos_3Germination HILIC Positive
SA193952Germinated_HILIC_Pos_2Germination HILIC Positive
SA193953Germinated_Lipids_Neg_4Germination Lipidomics (C8) Negative
SA193954Germinated_Lipids_Neg_1Germination Lipidomics (C8) Negative
SA193955Germinated_Lipids_Neg_2Germination Lipidomics (C8) Negative
SA193956Germinated_Lipids_Neg_3Germination Lipidomics (C8) Negative
SA193957Germinated_Lipids_Pos_4Germination Lipidomics (C8) Positive
SA193958Germinated_Lipids_Pos_2Germination Lipidomics (C8) Positive
SA193959Germinated_Lipids_Pos_1Germination Lipidomics (C8) Positive
SA193960Germinated_Lipids_Pos_3Germination Lipidomics (C8) Positive
SA193961Germinated_RPLC_Neg_3Germination RPLC (C18) Negative
SA193962Germinated_RPLC_Neg_4Germination RPLC (C18) Negative
SA193963Germinated_RPLC_Neg_2Germination RPLC (C18) Negative
SA193964Germinated_RPLC_Neg_1Germination RPLC (C18) Negative
SA193965Germinated_RPLC_Pos_1Germination RPLC (C18) Positive
SA193966Germinated_RPLC_Pos_4Germination RPLC (C18) Positive
SA193967Germinated_RPLC_Pos_3Germination RPLC (C18) Positive
SA193968Germinated_RPLC_Pos_2Germination RPLC (C18) Positive
SA193969Hydrated_HILIC_Neg_3Hydration HILIC Negative
SA193970Hydrated_HILIC_Neg_4Hydration HILIC Negative
SA193971Hydrated_HILIC_Neg_1Hydration HILIC Negative
SA193972Hydrated_HILIC_Neg_2Hydration HILIC Negative
SA193973Hydrated_HILIC_Pos_4Hydration HILIC Positive
SA193974Hydrated_HILIC_Pos_1Hydration HILIC Positive
SA193975Hydrated_HILIC_Pos_3Hydration HILIC Positive
SA193976Hydrated_HILIC_Pos_2Hydration HILIC Positive
SA193977Hydrated_Lipids_Neg_4Hydration Lipidomics (C8) Negative
SA193978Hydrated_Lipids_Neg_1Hydration Lipidomics (C8) Negative
SA193979Hydrated_Lipids_Neg_2Hydration Lipidomics (C8) Negative
SA193980Hydrated_Lipids_Neg_3Hydration Lipidomics (C8) Negative
SA193981Hydrated_Lipids_Pos_1Hydration Lipidomics (C8) Positive
SA193982Hydrated_Lipids_Pos_2Hydration Lipidomics (C8) Positive
SA193983Hydrated_Lipids_Pos_4Hydration Lipidomics (C8) Positive
SA193984Hydrated_Lipids_Pos_3Hydration Lipidomics (C8) Positive
SA193985Hydrated_RPLC_Neg_3Hydration RPLC (C18) Negative
SA193986Hydrated_RPLC_Neg_1Hydration RPLC (C18) Negative
SA193987Hydrated_RPLC_Neg_2Hydration RPLC (C18) Negative
SA193988Hydrated_RPLC_Neg_4Hydration RPLC (C18) Negative
SA193989Hydrated_RPLC_Pos_3Hydration RPLC (C18) Positive
SA193990Hydrated_RPLC_Pos_1Hydration RPLC (C18) Positive
SA193991Hydrated_RPLC_Pos_2Hydration RPLC (C18) Positive
SA193992Hydrated_RPLC_Pos_4Hydration RPLC (C18) Positive
SA193993Mature_HILIC_Neg_1Mature HILIC Negative
SA193994Mature_HILIC_Neg_2Mature HILIC Negative
SA193995Mature_HILIC_Neg_3Mature HILIC Negative
SA193996Mature_HILIC_Neg_4Mature HILIC Negative
SA193997Mature_HILIC_Pos_3Mature HILIC Positive
SA193998Mature_HILIC_Pos_2Mature HILIC Positive
SA193999Mature_HILIC_Pos_4Mature HILIC Positive
SA194000Mature_HILIC_Pos_1Mature HILIC Positive
SA194001Mature_Lipids_Neg_4Mature Lipidomics (C8) Negative
SA194002Mature_Lipids_Neg_3Mature Lipidomics (C8) Negative
SA194003Mature_Lipids_Neg_2Mature Lipidomics (C8) Negative
SA194004Mature_Lipids_Neg_1Mature Lipidomics (C8) Negative
SA194005Mature_Lipids_Pos_1Mature Lipidomics (C8) Positive
SA194006Mature_Lipids_Pos_3Mature Lipidomics (C8) Positive
SA194007Mature_Lipids_Pos_4Mature Lipidomics (C8) Positive
SA194008Mature_Lipids_Pos_2Mature Lipidomics (C8) Positive
SA194009Mature_RPLC_Neg_4Mature RPLC (C18) Negative
SA194010Mature_RPLC_Neg_3Mature RPLC (C18) Negative
SA194011Mature_RPLC_Neg_1Mature RPLC (C18) Negative
SA194012Mature_RPLC_Neg_2Mature RPLC (C18) Negative
SA194013Mature_RPLC_Pos_4Mature RPLC (C18) Positive
SA194014Mature_RPLC_Pos_3Mature RPLC (C18) Positive
SA194015Mature_RPLC_Pos_2Mature RPLC (C18) Positive
SA194016Mature_RPLC_Pos_1Mature RPLC (C18) Positive
Showing results 1 to 72 of 72
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