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MB Sample ID: SA084812
| Local Sample ID: | C5 |
| Subject ID: | SU001274 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6/J |
| Age Or Age Range: | 12 weeks old |
| Weight Or Weight Range: | 25-30 g |
| Gender: | Male |
| Animal Animal Supplier: | Jax Laboratories |
| Animal Housing: | Conventional |
| Animal Light Cycle: | regular 12h light /dark cycles |
| Animal Feed: | ad libitum normal chow diet |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002009 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ekspert MicroLC 200 system |
| Column | Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE |
| Units | Peak Area |
MS:
| MS ID: | MS001862 |
| Analysis ID: | AN002009 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX). C18SPE cartridges were purchased from Biotage. All solvents were of HPLC or LC-MS/MS grade and were acquired from Sigma-Aldrich, Fisher Scientific, or VWR International. |
| Ion Mode: | NEGATIVE |
