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MB Sample ID: SA256078
| Local Sample ID: | Uninfected-4 |
| Subject ID: | SU002649 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004197 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Waters Xevo-TQ-S |
| Ion Mode | UNSPECIFIED |
| Units | pmol/mL of plasma |
MS:
| MS ID: | MS003944 |
| Analysis ID: | AN004197 |
| Instrument Name: | Waters Xevo-TQ-S |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Lipid extraction and analysis for targeted sphingolipid analysis was done using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS)69. Lipids were extracted from sera using an azeotrophic mix of isopropanol:water:ethyl acetate (3:1:6; v:v:v). Internal standards (10 pmol of d17 long-chain bases and C12 acylated sphingolipids) were added to samples at the onset of the extraction procedure. Extracts were separated on a Waters Iclass Acquity UPLC chromatography system. Mobile phases were (A) 60:40 water:acetonitrile and (B) 90:10 isopropanol:methanol with both mobile phases containing 5 mM ammonium formate and 0.1% formic acid. A Waters C18 CSH 2.1 mm ID × 10 cm column maintained at 65°C was used for the separation of the sphingoid bases, 1-phosphates, and acylated sphingolipids. The eluate was analyzed with an inline Waters TQ-S mass spectrometer using multiple reaction monitoring. |
| Ion Mode: | UNSPECIFIED |
