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MB Sample ID: SA298619
| Local Sample ID: | HFD_HFD_HFD_504 |
| Subject ID: | SU002888 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6JRcc |
| Age Or Age Range: | 10-12 weeks |
| Animal Housing: | specific pathogen-free conditions |
| Animal Light Cycle: | 12-h light/dark cycle |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004528 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | Thermo Q Exactive Plus |
| Column | none |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | pmol/mg |
MS:
| MS ID: | MS004275 |
| Analysis ID: | AN004528 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Thermo Q Exactive Plus spectrometer equipped with the HESI II ion source. MS1 spectra (resolution 280 000) were recorded in 100 m/z windows from 250 to 1200 m/z (pos.) and 200 - 1700 m/z (neg.) followed by recording MS/MS spectra (res. 70 000) by data-independent acquisition in 1 m/z windows from 200 to 1200 (pos.) and 200 to 1700 (neg.) m/z. Raw files were converted to .mzml files and imported into and analyzed by LipidXplorer software using custom mfql files to identify sample lipids and internal standards. For further data processing, absolute amounts were calculated using the internal standard intensities followed by the calculated mol% of the identified lipids. |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Lipidomics_Notes.pdf |
