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MB Sample ID: SA328076
| Local Sample ID: | AS153 |
| Subject ID: | SU003142 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004964 | AN004965 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | SCIEX ExionLC AD | SCIEX ExionLC AD |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | count per second (cps) | count per second (cps) |
MS:
| MS ID: | MS004704 |
| Analysis ID: | AN004964 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | In this study, we performed extensive targeted detection of metabolites in the colon using MRM scanning modalities. Therefore, we did not perform MS/MS data annotation. In the MRM mode, the quadrupole firstly screens the precursor ion (parent ion) of the target substance and excludes the ions corresponding to other molecular weight substances in order to preliminarily exclude the interference; the precursor ion is induced to ionize by the collision chamber and breaks off to form a lot of fragment ions, and the fragment ions are then filtered through the triple quadrupole to select a desired characteristic fragment ion and exclude the interference of non-target ions. We used MultiQuant software to analyze the data. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS004705 |
| Analysis ID: | AN004965 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | In this study, we performed extensive targeted detection of metabolites in the colon using MRM scanning modalities. Therefore, we did not perform MS/MS data annotation. In the MRM mode, the quadrupole firstly screens the precursor ion (parent ion) of the target substance and excludes the ions corresponding to other molecular weight substances in order to preliminarily exclude the interference; the precursor ion is induced to ionize by the collision chamber and breaks off to form a lot of fragment ions, and the fragment ions are then filtered through the triple quadrupole to select a desired characteristic fragment ion and exclude the interference of non-target ions. We used MultiQuant software to analyze the data. |
| Ion Mode: | POSITIVE |
