Summary of Study ST000378
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000297. The data can be accessed directly via it's Project DOI: 10.21228/M8NP5K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000378 |
Study Title | 2 hydroxiglutarate with with IDH1-R132H inhibitor in neurospheres |
Study Type | Regular |
Study Summary | Tumor neurospheres carrying genetic lession NPI (NRAS, shP53, IDH1-R132H) colony 1 and 2 (C1 and C2); NPAI (NRAS, shP53, shATRX, IDH1-R132H) colony 1 and 2 (C1 and C2); and NPA (NRAS, shP53, shATRX) (C1 and C2). Was treated with IDH1-R132H inhibitor. By 2HG assay we want to check the effect of the inhibitor in our cells. |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@med.umich.edu | |
Phone | (734) 232-8175 |
Submit Date | 2016-04-20 |
Num Groups | 3 |
Total Subjects | 12 |
Study Comments | (shATRX) : ATRX knockdown sequence into a Sleeping Beauty (SB) transposase-responsive plasmid |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000612 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | None |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6410A QQQ |
Ion Mode | NEGATIVE |
Units | µmol/g |
MS:
MS ID: | MS000545 |
Analysis ID: | AN000612 |
Instrument Name: | Agilent 6410A QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | EX00452-2HG-QUANT-method.m.zip |