Summary of Study ST003167

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001970. The data can be accessed directly via it's Project DOI: 10.21228/M8314P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003167
Study TitleMetabolomics of Human CD19-BBz CD8 CAR-T cells with low and high MCJ expression
Study SummaryThe goal of these experiments are to characterize the metabolic profile of the human CD8 CD19-BBz CAR-T cells from donors with high or low MCJ expression after 3 expansions (6 days) with IL-2 (100IU/ml).
Institute
University of Colorado School of Medicine
LaboratoryLaboratory of Angelo D'Alessandro in collaboration with Mercedes Rincon
Last NameCendali
First NameFrancesca
Address13199 East Montview Boulevard, Aurora, CO, 80045, USA
Emailfrancesca.cendali@cuanschutz.edu
Phone3037246131
Submit Date2024-04-11
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-05-02
Release Version1
Francesca Cendali Francesca Cendali
https://dx.doi.org/10.21228/M8314P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005196 AN005197
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um)
MS Type ESI ESI
MS instrument type Exploris120 Exploris120
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units Peak Areas Peak Areas

MS:

MS ID:MS004929
Analysis ID:AN005196
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Exploris120
MS Type:ESI
MS Comments:Resolution 70,000, scan range 65-900 m/z, maximum injection time 200 ms, microscans 2, automatic gain control (AGC) 3 x 10^6 ions, source voltage 4.0 kV, capillary temperature 320 C, and sheath gas 45, auxiliary gas 15, and sweep gas 0 (all nitrogen). Data converted to mzXML using RawConverter. Metabolites were annotated and integrated using Maven in conjunction with the KEGG database.
Ion Mode:NEGATIVE
  
MS ID:MS004930
Analysis ID:AN005197
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Exploris120
MS Type:ESI
MS Comments:Resolution 70,000, scan range 65-900 m/z, maximum injection time 200 ms, microscans 2, automatic gain control (AGC) 3 x 10^6 ions, source voltage 4.0 kV, capillary temperature 320 C, and sheath gas 45, auxiliary gas 15, and sweep gas 0 (all nitrogen). Data converted to mzXML using RawConverter. Metabolites were annotated and integrated using Maven in conjunction with the KEGG database.
Ion Mode:POSITIVE
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