Summary of Study ST002284
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001464. The data can be accessed directly via it's Project DOI: 10.21228/M8GT5D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002284 |
| Study Title | Genetically defined human GBM organoids reveal principles of GBM development and actionable targets |
| Study Summary | Recent advances in glioblastoma (GBM) studies provide a comprehensive catalog of its genetic aberrations and cellular heterogeneity. However, a solid understanding of genotype-based analysis of cancer pathway dependency and actionable target identification is required to transform GBM treatment into a personalized era. Here, we generated a spectrum of mutant iPSCs harboring frequent GBM mutations with CRISPR/Cas9 and profiled the organoids (LEGO: Laboratory Engineered Glioblastoma Organoid) derived from these iPSCs temporally on transcriptome, methylome, metabolome, lipidome, proteome, and phospho-proteome levels. We found that LEGOs form brain tumors in vivo and recapitulate critical features of human GBM. The multi-omics analysis discovered essential milestones driven by genetic heterogeneity during GBM progressions, such as lineage alteration, methylome rewriting, and metabolome/lipidome reprogramming, in concordance with altered pathway activity and drug response. This study provides a tool and research path to realizing genome-based personalized GBM therapy using novel advanced models. |
| Institute | DKFZ |
| Department | Molecular Neurogenetics |
| Laboratory | Molecular Neurogenetics |
| Last Name | Wang |
| First Name | Changwen |
| Address | Im Neuenheimer Feld 581 |
| c.wang@dkfz-heidelberg.de | |
| Phone | +49 6221 42 3455 |
| Submit Date | 2022-09-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-26 |
| Release Version | 1 |
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Project:
| Project ID: | PR001464 |
| Project DOI: | doi: 10.21228/M8GT5D |
| Project Title: | Comparing water-soluble metabolites and lipid changes on human organoids and corresponding culture medium with different genotypes at different culturing stages |
| Project Summary: | Quantitative metabolome and lipidome analysis of human brain organoids with different genetic mutations. Water-soluble metabolites were extracted from 1- and 4-month-old WT, PT, PTCC, and PTN organoids and their respective culture medium. |
| Institute: | DKFZ |
| Department: | Molecular Neurogenetics |
| Laboratory: | Molecular Neurogenetics |
| Last Name: | Wang |
| First Name: | Changwen |
| Address: | Im Neuenheimer Feld 581, Heidelberg, Baden-Wurttemberg, 69120, Germany |
| Email: | c.wang@dkfz-heidelberg.de |
| Phone: | +49 6221 42 3455 |
| Publications: | https://www.nature.com/articles/s41698-024-00500-5, https://pubmed.ncbi.nlm.nih.gov/38273014/ |
