Summary of Study ST000622
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000454. The data can be accessed directly via it's Project DOI: 10.21228/M8CC9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000622 |
| Study Title | Identification and metabolite profiling of chemical activators of lipid accumulation in green algae |
| Study Type | GC-MS metabolite profiling of algal lipid activators |
| Study Summary | Microalgae are proposed as feedstock organisms useful for producing biofuels and co-products. However, several limitations must be overcome before algae-based production is economically feasible. Among these is the ability to induce lipid accumulation and storage without affecting biomass yield. To overcome this barrier, a chemical genetics approach was employed in which 43,783 compounds were screened against Chlamydomonas reinhardtii and 243 compounds were identified that increase triacylglyceride (TAG) accumulation without terminating growth. Identified compounds were classified by structural similarity and 15 selected for secondary analyses addressing impacts on growth fitness, photosynthetic pigments, and total cellular protein and starch concentrations. TAG accumulation was verified using GC-MS quantification of total fatty acids and targeted TAG and galactolipid (GL) measurements using LC-MRM/MS. These results demonstrated TAG accumulation does not necessarily proceed at the expense of GL. Untargeted metabolite profiling provided important insights into pathway shifts due to 5 different compound treatments and verified the anabolic state of the cells with regard to the oxidative pentose phosphate pathway, Calvin cycle, tricarboxylic acid cycle and amino acid biosynthetic pathways. Metabolite patterns were distinct from nitrogen starvation and other abiotic stresses commonly used to induce oil accumulation in algae. The efficacy of these compounds was also demonstrated in 3 other algal species. These lipid inducing compounds offer a valuable set of tools for delving into the biochemical mechanisms of lipid accumulation in algae and a direct means to improve algal oil content independent of the severe growth limitations associated with nutrient deprivation. |
| Institute | University of Nebraska-Lincoln |
| Department | Biochemistry |
| Laboratory | FATTTLab |
| Last Name | Wase |
| First Name | Nishikant |
| Address | 1901 Beadle Center, Vine Street, 1901 VINE STREET, Lincoln, NE, 68588-0664, USA |
| nishikant.wase@gmail.com | |
| Phone | 4023109931 |
| Submit Date | 2017-06-16 |
| Num Groups | 6 |
| Publications | 1. Nishikant Wase, Boqiang Tu, James W Allen, Paul N Black, Concetta C DiRusso. Identification and metabolite profiling of chemical activators of lipid accumulation in green algae. Plant Physiology Jun 2017. DOI: 10.1104/pp.17.00433. http://www.plantphysiol.org/content/early/2017/06/26/pp.17.00433; 2. DiRusso, C., & Wase, N. (2016). Compounds for Increasing Lipid Synthesis and Storage. United States. NUtech Ventures (Lincoln, NE, US) http://www.freepatentsonline.com/y2016/0312253.html |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2017-10-03 |
| Release Version | 1 |
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Project:
| Project ID: | PR000454 |
| Project DOI: | doi: 10.21228/M8CC9H |
| Project Title: | Untargeted metabolomic changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules |
| Project Summary: | A study to investigate the effect of small molecule lipid inducing compounds that leads to hyper accumulation of lipids in N replete cells of Chlamydomonas reinhardtii. These compounds were identified through a high throughput screening designed for that purpose. During that screening, we screened 43,783 compounds and identified 367 primary hits. These 367 hits were further retested using a 8-point dilution series (from 0.25 to 30 uM) and verified the activity of 250 compounds that induce the hyper lipid accumulating phenotype in algae. Once the hit compounds were identified and confirmed, we then performed extensive chemoinformatics analysis to look for common scaffolds and identified several common substructures. We then selected 15 top performing compounds from 5 diverse structural groups and tested biochemical parameters such as growth, lipid accumulating capacity, effect on photosynthetic rates, respiration rates, oxygen consumption rates, analysis of different lipid species to quantify and identify fatty acid species using GC-MS. To understand the global changes in the metabolome, 2 structurally different compounds were selected and compared with cells grown without compounds as control for untargeted metabolomics analysis. |
| Institute: | University of Nebraska-Lincoln |
| Department: | Biochemistry |
| Laboratory: | FATTTLab |
| Last Name: | Wase |
| First Name: | Nishikant |
| Address: | 1901 Beadle Center, Vine Street, 1901 VINE STREET, Lincoln, NE, 68588-0664, USA |
| Email: | nishikant.wase@gmail.com |
| Phone: | 4023109931 |
| Funding Source: | NCESR-704, Nebraska Center for Energy Science Research; EPS-1004094 and 1264409, National Science Foundation ; NSF CBET : 1402896, National Science Foundation |
| Contributors: | Nishikant Wase, Jiri Adamec, Ron Cerny, Girish Rasineni, Paul N Black, Concetta DiRusso |
