Summary of Study ST003168
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001927. The data can be accessed directly via it's Project DOI: 10.21228/M8N42X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003168 |
| Study Title | Lipidomic analysis of cryopreserved human cardiac tissue from young and ageing adults |
| Study Summary | Untargeted lipidomic analysis was performed to measure lipids in left ventricular heart tissue from pre-mortem healthy donor hearts, as classified by formal pathological examination. Hearts were stored at the Sydney Heart Bank. Samples were divided into young (age ≤ 25 years) and old (age ≥ 50 years) cohorts. Lipidomic analysis used liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a high resolution Q-Exactive HF-X Quadrupole-Orbitrap mass spectrometer, operated in both positive and negative ionisation mode. |
| Institute | University of Sydney |
| Department | Medicine and Health |
| Laboratory | Lipid Metabolism and Neurochemistry |
| Last Name | Don |
| First Name | Anthony |
| Address | The Hub, Charles Perkins Centre, D17, The University of Sydney, NSW, 2006 |
| anthony.don@sydney.edu.au | |
| Phone | +61 2 8627 5578 |
| Submit Date | 2024-03-20 |
| Num Groups | 2 |
| Total Subjects | 23 |
| Num Males | 15 |
| Num Females | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-05-03 |
| Release Version | 1 |
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Project:
| Project ID: | PR001927 |
| Project DOI: | doi: 10.21228/M8N42X |
| Project Title: | The Human Cardiac “Age-OME”: Age-specific changes in myocardial molecular expression |
| Project Type: | MS analysis |
| Project Summary: | A substantial proportion of the World’s population is ageing. One of the most significant risk factors for heart disease is ageing. However, we do not understand how the human heart changes with age. Taking advantage of a unique set of pre-mortem, cryopreserved, non-diseased human hearts, we performed multi-omic analyses (transcriptomics, proteomics, metabolomics and lipidomics), coupled with biological computational modelling in younger (<25 years old) and older hearts (>50years old) to describe the molecular landscape of human cardiac ageing. In older hearts, we observed a downregulation of proteins involved in calcium signalling and of the contractile apparatus itself. In addition, we found a potential counteractive upregulation of central carbon generation of fuel, upregulation of glycolysis and increases in long-chain fatty acids. This is the first molecular data set of normal human cardiac ageing, which may have important implications for the development of age-related heart disease. |
| Institute: | University of Sydney |
| Department: | School of Medical Sciences |
| Laboratory: | Cardiometabolic Medicine |
| Last Name: | Koay |
| First Name: | Yen Chin |
| Address: | The Hub, Charles Perkins Centre, D17, The University of Sydney, NSW, 2006 |
| Email: | yen.koay@sydney.edu.au |
| Phone: | +61486275851 |
