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MB Sample ID: SA012099

Local Sample ID:QE1_REP_18_27
Subject ID:SU000289
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Age Or Age Range:8 or 24 week
Gender:male
Animal Animal Supplier:Jackson Laboratories
Animal Feed:Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301)
Species Group:Mammal

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Sample Preparation:

Sampleprep ID:SP000297
Sampleprep Summary:Tissues were cryogenically ground prior to beign weighed and homogenized in chloroform and methanol for the Folch extraction
Processing Method:homogenization (zirconium beads, 1.0 mm diameter), lipid extraction
Processing Storage Conditions:Liquid nitrogen and on ice
Extraction Method:Folch
Extract Storage:-80C
Sample Resuspension:isopropanol
Sample Spiking:Internal Standards: Cer(d18:1/17:0) and d5-DAG (20:2/20:2) and d5-DG (20:5/20:5)
Organ:liver
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