Return to study ST001207 main page

MB Sample ID: SA084810

Local Sample ID:C4
Subject ID:SU001274
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL6/J
Age Or Age Range:12 weeks old
Weight Or Weight Range:25-30 g
Gender:Male
Animal Animal Supplier:Jax Laboratories
Animal Housing:Conventional
Animal Light Cycle:regular 12h light /dark cycles
Animal Feed:ad libitum normal chow diet
Animal Water:ad libitum

Select appropriate tab below to view additional metadata details:


Sample Preparation:

Sampleprep ID:SP001282
Sampleprep Summary:Aliquots of 100 µL serum were taken and a mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform.
  logo