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MB Sample ID: SA113597
Local Sample ID: | KO_AZA_2 |
Subject ID: | SU001473 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Samtako (Gyeonggi-do, Korea) |
Gender: | Male |
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Sample Preparation:
Sampleprep ID: | SP001481 |
Sampleprep Summary: | Methoximation and silylation were performed for qualification and relative quantification of metabolites using GC/TOF–MS, For methoximation, 10 µL of 40 mg/mL methoxyamine hydrochloride in pyridine (Sigma-Aldrich) was added to the dried sample, and the mixture was incubated at 30°C for 90 minutes. For silylation, 45 µL of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) was added to the mixture, and the mixture was incubated at 37°C for 30 minutes. |